PXD014639

PXD014639 is an original dataset announced via ProteomeXchange.

Title	ER-shaping Atlastin proteins differentially contribute to flavivirus replication and assembly
Description	Flaviviruses, including Dengue virus (DV) and Zika virus, extensively remodel the cellular endomembrane network to generate replication organelles that promote viral genome replication and virus production. However, it remains unclear how these membranes and associated cellular proteins act during the virus cycle. Here, we show that atlastins (ATLs), a subset of ER resident proteins involved in neurodegenerative diseases, have dichotomous effects on flaviviruses with ATL2 depletion leading to replication organelle defects and ATL3 depletion to changes in virus production pathways. We characterized non-conserved functional domains in ATL paralogues and show that the ATL interactome is profoundly reprogrammed upon DV infection. Screen analysis confirmed non-redundant ATL functions and identified a specific role for ATL3, and its interactor ARF4, in vesicle trafficking and virion maturation. Our data identify ATLs as central hubs targeted by flaviviruses to establish their replication organelle and to achieve efficient virion maturation and secretion.
HostingRepository	PRIDE
AnnounceDate	2019-10-17
AnnouncementXML	Submission_2019-10-25_06:43:44.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pietro Scaturro
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-16 23:26:13	ID requested
1	2019-10-17 08:44:16	announced
⏵ 2	2019-10-25 06:43:46	announced	2019-10-25: Updated publication reference for PubMed record(s): 31636417.

Neufeldt CJ, Cortese M, Scaturro P, Cerikan B, Wideman JG, Tabata K, Moraes T, Oleksiuk O, Pichlmair A, Bartenschlager R, ER-shaping atlastin proteins act as central hubs to promote flavivirus replication and virion assembly. Nat Microbiol, 4(12):2416-2429(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Atlastin, ATL-2, ATL-3, proteome, AP-LC-MS/MS

Ralf Bartenschlager
contact affiliation	Department of Infectious Diseases, Molecular Virology, Heidelberg University, Im Neuenheimer Feld 344, Heidelberg, Germany
contact email	ralf.bartenschlager@med.uni-heidelberg.de
lab head
Pietro Scaturro
contact affiliation	Technical University Munich, Department of Virology
contact email	pietro.scaturro@tum.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014639

PRIDE project URI

• PRIDE
  1. PXD014639
     1. Label: PRIDE project
     2. Name: ER-shaping Atlastin proteins differentially contribute to flavivirus replication and assembly