PXD014629

PXD014629 is an original dataset announced via ProteomeXchange.

Title	Quantitative analysis of in vivo methionine oxidation of the human proteome
Description	A novel stable isotope labelling strategy was developed to quantify methionine oxidation in an unstressed human proteome. Cell extracts were oxidized with 18O labelled hydrogen peroxide following cell lysis in order to convert all unoxidized methionine residues to an oxidized version with a heavy label. The heavy labelled peptides were used to generate a custom search and quantification of the relative ratios between heavy and light labelled methionine sulfoxide containing peptides. Where the light labelled peptides were in vivo oxidized
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_03:58:55.836.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John Bettinger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-16 02:20:48	ID requested
1	2019-12-09 02:41:03	announced
⏵ 2	2024-10-22 03:58:59	announced	2024-10-22: Updated project metadata.

Bettinger JQ, Welle KA, Hryhorenko JR, Ghaemmaghami S, Quantitative Analysis of in Vivo Methionine Oxidation of the Human Proteome. J Proteome Res, 19(2):624-633(2020) [pubmed]

10.1021/acs.jproteome.9b00505;

submitter keyword: methionine oxidation, unstressed proteome, human cell line

Sina Ghaemmaghami
contact affiliation	University of Rochester Biology Department
contact email	sina.ghaemmaghami@rochester.edu
lab head
John Bettinger
contact affiliation	University of Rochester
contact email	jbetting@ur.rochester.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014629

PRIDE project URI

• PRIDE
  1. PXD014629
     1. Label: PRIDE project
     2. Name: Quantitative analysis of in vivo methionine oxidation of the human proteome