PXD014624

PXD014624 is an original dataset announced via ProteomeXchange.

Title	Mechanical forces regulate cardiomyocyte myofilament maturation via the VCL-SSH1-CFL axis
Description	Mechanical forces regulate cell behavior and tissue morphogenesis. In particular, cardiac tissues require mechanical stimuli generated by the heartbeat for differentiation and maturation, but the molecular mechanisms underlying these processes remain unclear. Here, we first show that mechanical forces acting via the mechanosensitive factor Vinculin (VCL) are essential for cardiomyocyte myofilament maturation and that cardiac contractility regulates the localization and activation of Vinculin. To further analyze the role of Vinculin in myofilament maturation, we examined its interactome in contracting cardiomyocytes and found many cytoskeletal factors including actinins. We also identified Slingshot protein phosphatase 1 (SSH1), which we show is recruited by Vinculin to regulate F-actin rearrangement and myofilament maturation through its association with the actin depolymerizing factor Cofilin (CFL). Together, our results reveal that mechanical forces generated by cardiac contractility regulate cardiomyocyte maturation through the VCL-SSH1-CFL axis, providing mechanistic insight into how mechanical forces are transmitted intracellularly to regulate myofilament maturation.
HostingRepository	PRIDE
AnnounceDate	2021-10-29
AnnouncementXML	Submission_2021-10-29_04:29:45.823.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Johannes Graumann
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-16 00:10:20	ID requested
1	2019-09-06 02:14:02	announced
2	2019-10-10 08:42:47	announced	2019-10-10: Updated project metadata.
⏵ 3	2021-10-29 04:29:46	announced	2021-10-29: Updated project metadata.

Fukuda R, Gunawan F, Ramadass R, Beisaw A, Konzer A, Mullapudi ST, Gentile A, Maischein HM, Graumann J, Stainier DYR, Mechanical Forces Regulate Cardiomyocyte Myofilament Maturation via the VCL-SSH1-CFL Axis. Dev Cell, 51(1):62-77.e5(2019) [pubmed]

curator keyword: Biological

submitter keyword: heart,Rattus norvegicus, cardiac contractility

Didier Y.R. Stainier
contact affiliation	Max-Planck-Institute for Heart and Lung Research, Bad Nauheim, Germany
contact email	didier.stainier@mpi-bn.mpg.de
lab head
Johannes Graumann
contact affiliation	Max Planck Institute for Heart and Lung Research
contact email	johannes.graumann@mpi-bn.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014624
     1. Label: PRIDE project
     2. Name: Mechanical forces regulate cardiomyocyte myofilament maturation via the VCL-SSH1-CFL axis