PXD014603

PXD014603 is an original dataset announced via ProteomeXchange.

Title	A Comprehensive Analysis of the Lysine Acetylome Reveals Diverse Functions of Acetylated Proteins During De-etiolation in Zea mays
Description	Lysine acetylation is one of the most important post-translational modifications and is involved in multiple cellular processes in plants. There is evidence that acetylation may play an important role in light-induced de-etiolation, a key developmental switch from skotomorphogenesis to photomorphogenesis. During this transition, establishment of photosynthesis is of great significance. However, studies on acetylome dynamics during de-etiolation are limited. Here, we performed the first global lysine acetylome analysis for Zea mays seedlings undergoing de-etiolation, using nano liquid chromatography coupled to tandem mass spectrometry, and identified 875 lysine acetylated sites on 494 proteins. Bioinformatics analysis of this acetylome showed that most of the lysine acetylated proteins are predicted to be located in the cytoplasm, nucleus, chloroplast and mitochondria. In addition, we detected ten lysine acetylation motifs and found that the accumulation of 482 lysine acetylated peptides corresponding to 289 proteins changed significantly during de-etiolation. These proteins include transcription factors, histones, and proteins involved in chlorophyll synthesis, photosynthesis light reaction, carbon assimilation, glycolysis, the TCA cycle, amino acid metabolism, lipid metabolism and nucleotide metabolism. Our study provides an in-depth dataset that extends our knowledge of in vivo acetylome dynamics during greening in monocots. This dataset promotes our understanding of the functional consequences of lysine acetylation in diverse cellular metabolic regulatory processes, and will be a useful toolkit for further investigations of the lysine acetylome and de-etiolation in plants.
HostingRepository	iProX
AnnounceDate	2019-07-14
AnnouncementXML	Submission_2020-12-01_23:07:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tiancong Lu
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-14 19:53:42	ID requested
1	2019-07-14 19:56:40	announced
2	2019-07-16 02:35:55	announced	Update information.
⏵ 3	2020-12-01 23:07:56	announced	2020-12-02: Update publication information

Yan Z, Shen Z, Gao ZF, Chao Q, Qian CR, Zheng H, Wang BC, A comprehensive analysis of the lysine acetylome reveals diverse functions of acetylated proteins during de-etiolation in Zea mays. J Plant Physiol, 248():153158(2020) [pubmed]

submitter keyword: Lysine-acetylation, De-etiolation, Maize seedling, Photosynthesis establishment

Baichen Wang
contact affiliation	Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
contact email	wangbc@ibcas.ac.cn
lab head
Tiancong Lu
contact affiliation	Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences
contact email	proteomelandscape@163.com
dataset submitter

iProX dataset URI