PXD014592

PXD014592 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Epicardial Adipose Tissue in Patients with Heart Failure
Description	Epicardial adipose tissue (EAT) is a metabolically active visceral fat depot closely linked to the pathogenesis of heart failure (HF). But the molecular signatures related to the mechanism of HF have not been systematically explored. Here, we present comprehensive proteomic analysis of EAT in HF patients and non-HF subjects as controls. A total of 771 proteins were identified in liquid chromatography-tandem mass spectrometry experiments. Among them, 17 increased in abundance in HF and 7 decreased. They were involved in HF-related processes including inflammation and oxidative stress response and lipid metabolism. Of these proteins, Serine proteinase inhibitor A3 (Serpina3) levels in EAT were highly upregulated in HF, with HF/non-HF ratio of 4.63 and a P value of 0.0047. Gene expression of Serpina3 via quantitative polymerase chain reaction was significantly increased in the HF group. ELISA analysis confirmed a significant increase in circulating plasma Serpina3 levels in the HF group (P = 0.004). In summary, for the first time we describe EAT proteome as a possible trigger for HF. Our profiling provides a comprehensive basis for linking EAT with pathogenesis of HF. Understanding the role of EAT may offer new insights into the treatment of HF.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_14:36:47.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Zhao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-12 07:31:08	ID requested
⏵ 1	2020-05-26 14:36:48	announced

Zhao L, Guo Z, Wang P, Zheng M, Yang X, Liu Y, Ma Z, Chen M, Yang X, Proteomics of epicardial adipose tissue in patients with heart failure. J Cell Mol Med, 24(1):511-520(2020) [pubmed]

submitter keyword: Heart failure

Epicardial adipose tissue

Proteomics

Xinchun Yang
contact affiliation	Beijing Chaoyang Hospital, Capital Medical University
contact email	yangxinchun6229@163.com
lab head
Lei Zhao
contact affiliation	Beijing Chaoyang Hospital
contact email	lily885300@sina.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014592

PRIDE project URI

• PRIDE
  1. PXD014592
     1. Label: PRIDE project
     2. Name: Proteomics of Epicardial Adipose Tissue in Patients with Heart Failure