PXD014590

PXD014590 is an original dataset announced via ProteomeXchange.

Title	Investigation of phycobilisome degradation using top down proteomics in Synechococcus elongatus UTEX 2973
Description	Top down mass spectrometry analysis of Synechococcus elongatus UTEX2973 cyanobacteria under nitrogen starvation over time course of 4 hours. A number of phycobiliproteins covalently linked to phycocyanobilin pigments were detected as intact forms. Some of them showed partial degradation of the protein backbones or the pigment structures. NblA, a small protein that is believed to play a key role in phycobilisome degradation was shown to be highly correlated to nitrogen starvation based on its remarkable change of protein abundance.
HostingRepository	PRIDE
AnnounceDate	2019-08-27
AnnouncementXML	Submission_2019-08-27_00:53:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matthew Monroe
SpeciesList	scientific name: Synechococcus elongatus; NCBI TaxID: 32046;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-12 06:01:28	ID requested
⏵ 1	2019-08-27 00:53:02	announced

Nagarajan A, Zhou M, Nguyen AY, Liberton M, Kedia K, Shi T, Piehowski P, Shukla A, Fillmore TL, Nicora C, Smith RD, Koppenaal DW, Jacobs JM, Pakrasi HB, UTEX 2973. Biomolecules, 9(8):(2019) [pubmed]

submitter keyword: Phycobilisomes, protein degradation, light harvesting complexes, nitrogen starvation, top down proteomics, photosynthesis

Himadri B Pakrasi
contact affiliation	Washington University in St. Louis
contact email	pakrasi@wustl.edu
lab head
Matthew Monroe
contact affiliation	Pacific Northwest National Laboratory, USA
contact email	matthew.monroe@pnnl.gov
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/08/PXD014590

PRIDE project URI

• PRIDE
  1. PXD014590
     1. Label: PRIDE project
     2. Name: Investigation of phycobilisome degradation using top down proteomics in Synechococcus elongatus UTEX 2973