PXD014588

PXD014588 is an original dataset announced via ProteomeXchange.

Title	Biological strategies for mitigating Escherichia coli enterohemorragic (EHEC) colonization in meat and meat products
Description	The enterohemorrhagic Escherichia (E.) coli (EHEC) is a pathogen of great concern for public health and the meat industry all over the world. High economic losses in meat industry and the high cost of the illness evidence the necessity of additional efforts to control this pathogen. Previous studies demonstrated inhibitory activity towards EHEC, of a bioprotective strain, Enterococcus mundtii CRL35, it showing also a specific proteomic response during the co-culture. In the present work additional studies of the EHEC-Ent. mundtii interaction were carried out: i) differential protein expression of E. coli O157:H7 NCTC12900 when growing in co-culture with Enterococcus mundtii in a meat environment, ii) the reciprocal influence between these two microorganisms in the adhesion to extracellular matrix (ECM) proteins and iii) the possible induction of the phage W933, coding for Shiga toxin (Stx1), by the presence of Ent. mundtii CRL35. When compared the co-culture with individual growth, proteomic results showed significant repression of E. coli NCTC12900 proteins related mostly to the metabolism and transport of amino acids and nucleotides. However, statistically significant over expression of EHEC proteins involved in stress, energy production, amino acid metabolism and transcription was observed at 30 h respect to 6 h when EHEC grew in co-culture. On the other hand, EHEC showed a decreased adhesion capacity to ECM proteins in the presence of the bioprotective strain. Finally, Ent. mundtii CRL35 did not induce the lytic cycle of W933 bacteriophage, thus indicating its potential safe use for eliminating this pathogen. Overall, this study expands the knowledge of EHEC- Ent. mundtii CRL35 interaction in a meat environment, as an attempt to find out effective biological strategies to eliminate this pathogen.
HostingRepository	PRIDE
AnnounceDate	2019-09-30
AnnouncementXML	Submission_2019-09-30_01:48:26.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sebastien Planchon
SpeciesList	scientific name: Escherichia coli O157:H7; NCBI TaxID: 83334; scientific name: Enterococcus mundtii CRL35; NCBI TaxID: 1450511;
ModificationList	dihydroxylated residue; methylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	5800 TOF/TOF; ultraflex TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-12 02:11:36	ID requested
⏵ 1	2019-09-30 01:48:27	announced

Orihuel A, Ter, á, n L, Renaut J, Planchon S, Valacco MP, Masias E, Minahk C, Vignolo G, Moreno S, De Almeida AM, Saavedra L, Fadda S, Physiological and proteomic response of Escherichia coli O157:H7 to a bioprotective lactic acid bacterium in a meat environment. Food Res Int, 125():108622(2019) [pubmed]

submitter keyword: ESCHERICHIA COLI, LACTIC ACID BACTERIA, MEAT PROTEOMICS, BACTERIAL INTERACTION, BIOPROTECTION

Dr. Silvina Fadda
contact affiliation	CERELA CONICET - Argentina (Reference Center for Lactobacilli, National Scientific and Technical Research Council - Argentina)
contact email	sfadda@cerela.org.ar
lab head
Sebastien Planchon
contact affiliation	Luxembourg Institute of Science and Technology
contact email	sebastien.planchon@list.lu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014588

PRIDE project URI

• PRIDE
  1. PXD014588
     1. Label: PRIDE project
     2. Name: Biological strategies for mitigating Escherichia coli enterohemorragic (EHEC) colonization in meat and meat products