PXD014580

PXD014580 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomics profiling of NSPs in Leishmania mexicana parasites during starvation
Description	Using a combination of BONCAT technique and iTRAQ 4-plex quantitative proteomics MS, we profiled the de novo protein synthesis in the starving L. mexicana parasites in a temporally resolved manner. This approach uniquely integrates the high specificity of the BONCAT technique for the NSPs with the high sensitivity and multiplexed quantitation capability of the iTRAQ quantitative proteomics MS. Proof-of-concept experiments identified 166 NSPs in the parasite and quantified the relative changes in abundance of these proteins as a function of duration of starvation. Our results show a starvation time-dependent differential expression of important translation regulators in the parasite.
HostingRepository	PRIDE
AnnounceDate	2019-12-03
AnnouncementXML	Submission_2020-01-14_23:51:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kalesh Karunakaran
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-11 07:59:38	ID requested
1	2019-12-03 00:09:33	announced
⏵ 2	2020-01-14 23:51:28	announced	2020-01-15: Updated publication reference for PubMed record(s): 31856154.

Kalesh K, Denny PW, A BONCAT-iTRAQ method enables temporally resolved quantitative profiling of newly synthesised proteins in Leishmania mexicana parasites during starvation. PLoS Negl Trop Dis, 13(12):e0007651(2019) [pubmed]

submitter keyword: Leishmania mexicana, Traslation, BONCAT, iTRAQ, proteomics

Kalesh Karunakaran
contact affiliation	Department of Chemistry, Durham University, Stockton Road, Durham - DH1 3LE, UK
contact email	kalesh.karunakaran@durham.ac.uk
lab head
Kalesh Karunakaran
contact affiliation	Durham University
contact email	kalesh.karunakaran@durham.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014580

PRIDE project URI

• PRIDE
  1. PXD014580
     1. Label: PRIDE project
     2. Name: Quantitative proteomics profiling of NSPs in Leishmania mexicana parasites during starvation