PXD014578

PXD014578 is an original dataset announced via ProteomeXchange.

Title	Rhomboid mediates membrane protein quality control as an intramembrane protease and adaptor of AAA protease.
Description	Magnesium homeostasis is essential for life and depends on magnesium transporters whose activity and ion selectivity need to be tightly controlled. Rhomboid intramembrane proteases pervade the prokaryotic kingdom but their functions are largely elusive. Using proteomics we find that Bacillus subtilis rhomboid protease YqgP interacts with the membrane bound ATP-dependent processive metalloprotease FtsH and cleaves MgtE, the major high-affinity magnesium transporter in B. subtilis. MgtE cleavage by YqgP is potentiated in low magnesium and high manganese or zinc conditions, which protects B. subtilis from Mn2+/ Zn2+ toxicity. The N-terminal cytosolic domain of YqgP binds Mn2+ and Zn2+ and facilitates MgtE cleavage. Independently of its protease activity, YqgP acts as a substrate adaptor for FtsH, which is necessary for degradation of MgtE. YqgP thus unites a protease and pseudoprotease function, which indicates the evolutionary origin of rhomboid pseudoproteases, such as Derlins that are intimately involved in ER associated degradation. Conceptually, the YqgP-FtsH system we describe here is equivalent to a primordial form of ‘ERAD’ in bacteria and exemplifies an ancestral function of rhomboid-superfamily proteins.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:30:37.920.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jana Brezinova
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	6x(13)C; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-11 07:59:32	ID requested
⏵ 1	2024-10-07 13:30:38	announced

Began J, Cordier B, B, ř, ezinov, á J, Delisle J, Hexnerov, á R, Srb P, Ramp, í, rov, á P, Ko, ž, í, š, ek M, Baudet M, Cout, é Y, Galinier A, Veverka V, Doan T, Strisovsky K, Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease. EMBO J, 39(10):e102935(2020) [pubmed]

10.15252/embj.2019102935;

submitter keyword: transporter, ER associated degradation,Intramembrane protease, manganese, TripleTOF 5600, magnesium, Bacillus subtilis, lipid membrane, GeLC-MS/MS, rhomboid protease, proteostasis, zinc

Kvido Strisovsky
contact affiliation	Institute of Organic Chemistry and Biochemistry, Czech Academy of Science, Flemingovo n. 2, Prague, 166 10, Czech Republic
contact email	kvido.strisovsky@uochb.cas.cz
lab head
Jana Brezinova
contact affiliation	Institute of Organic Chemistry and Biochemistry, Czech Academy of Science, Flemingovo n. 2, Prague, 166 10, Czech Republic
contact email	jana.brezinova@uochb.cas.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014578
     1. Label: PRIDE project
     2. Name: Rhomboid mediates membrane protein quality control as an intramembrane protease and adaptor of AAA protease.