PXD014566
PXD014566 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of candidate interactors of Bacillus subtilis rhomboid protease YqgP |
Description | Magnesium homeostasis is essential for life and depends on magnesium transporters whose activity and ion selectivity needs to be tightly controlled. Rhomboid intramembrane proteases pervade the prokaryotic kingdom but their functions are largely elusive. Using proteomics we find that Bacillus subtilis rhomboid protease YqgP interacts with the membrane bound ATP-dependent processive metalloprotease FtsH and cleaves MgtE, the major high-affinity magnesium transporter in B. subtilis. MgtE cleavage by YqgP is potentiated in low magnesium and high manganese or zinc conditions, which protects B. subtilis from Mn2+/ Zn2+ toxicity. The N-terminal cytosolic domain of YqgP binds Mn2+ and Zn2+ and facilitates MgtE cleavage. Independently of its protease activity, YqgP acts as a substrate adaptor for FtsH, which is necessary for degradation of MgtE. YqgP thus unites a protease and pseudoprotease function, which indicates the evolutionary origin of rhomboid pseudoproteases, such as Derlins that are intimately involved in ER associated degradation. Conceptually, the YqgP-FtsH system we describe here is equivalent to a primordial form of ‘ERAD’ in bacteria and exemplifies an ancestral function of rhomboid-superfamily proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-09 |
AnnouncementXML | Submission_2020-04-14_03:05:37.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD014566 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yohann Couté |
SpeciesList | scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308; |
ModificationList | Deamidated; Oxidation; Acetyl; Carbamidomethyl |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-07-11 01:06:39 | ID requested | |
1 | 2020-01-09 10:58:33 | announced | |
⏵ 2 | 2020-04-14 03:05:39 | announced | 2020-04-14: Updated publication reference for PubMed record(s): 31930742. |
Publication List
Began J, Cordier B, B, ř, ezinov, á J, Delisle J, Hexnerov, á R, Srb P, Ramp, í, rov, á P, Ko, ž, í, š, ek M, Baudet M, Cout, é Y, Galinier A, Veverka V, Doan T, Strisovsky K, Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease. EMBO J, 39(10):e102935(2020) [pubmed] |
Keyword List
submitter keyword: Intramembrane protease, rhomboid protease, lipid membrane, proteostasis, transporter, co-immunoprecipitation, LC-MS/MS |
Contact List
Virginie Brun | |
---|---|
contact affiliation | EDyP |
contact email | virginie.brun@cea.fr |
lab head | |
Yohann Couté | |
contact affiliation | EDyP |
contact email | yohann.coute@cea.fr |
dataset submitter |
Full Dataset Link List
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PRIDE project URI |
Repository Record List
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