PXD014566

PXD014566 is an original dataset announced via ProteomeXchange.

Title	Identification of candidate interactors of Bacillus subtilis rhomboid protease YqgP
Description	Magnesium homeostasis is essential for life and depends on magnesium transporters whose activity and ion selectivity needs to be tightly controlled. Rhomboid intramembrane proteases pervade the prokaryotic kingdom but their functions are largely elusive. Using proteomics we find that Bacillus subtilis rhomboid protease YqgP interacts with the membrane bound ATP-dependent processive metalloprotease FtsH and cleaves MgtE, the major high-affinity magnesium transporter in B. subtilis. MgtE cleavage by YqgP is potentiated in low magnesium and high manganese or zinc conditions, which protects B. subtilis from Mn2+/ Zn2+ toxicity. The N-terminal cytosolic domain of YqgP binds Mn2+ and Zn2+ and facilitates MgtE cleavage. Independently of its protease activity, YqgP acts as a substrate adaptor for FtsH, which is necessary for degradation of MgtE. YqgP thus unites a protease and pseudoprotease function, which indicates the evolutionary origin of rhomboid pseudoproteases, such as Derlins that are intimately involved in ER associated degradation. Conceptually, the YqgP-FtsH system we describe here is equivalent to a primordial form of ‘ERAD’ in bacteria and exemplifies an ancestral function of rhomboid-superfamily proteins.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-04-14_03:05:37.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014566
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Bacillus subtilis subsp. subtilis str. 168; NCBI TaxID: 224308;
ModificationList	Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-11 01:06:39	ID requested
1	2020-01-09 10:58:33	announced
⏵ 2	2020-04-14 03:05:39	announced	2020-04-14: Updated publication reference for PubMed record(s): 31930742.

Began J, Cordier B, B, ř, ezinov, á J, Delisle J, Hexnerov, á R, Srb P, Ramp, í, rov, á P, Ko, ž, í, š, ek M, Baudet M, Cout, é Y, Galinier A, Veverka V, Doan T, Strisovsky K, Rhomboid intramembrane protease YqgP licenses bacterial membrane protein quality control as adaptor of FtsH AAA protease. EMBO J, 39(10):e102935(2020) [pubmed]

submitter keyword: Intramembrane protease, rhomboid protease, lipid membrane, proteostasis, transporter, co-immunoprecipitation, LC-MS/MS

Virginie Brun
contact affiliation	EDyP
contact email	virginie.brun@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014566
     1. Label: PRIDE project
     2. Name: Identification of candidate interactors of Bacillus subtilis rhomboid protease YqgP