PXD014550

PXD014550 is an original dataset announced via ProteomeXchange.

Title	The secretome of the rumen fluke, Calicophoron daubneyi
Description	Helminth parasites secrete molecules in soluble form (i.e the secretome) or packaged into extracellular vesicles (EVs) as a means of exporting effector molecules into the host microenvironment. Once released, the parasite-derived molecules can trigger a range of biological effects including modulation of host immunity. While the secretome and molecular cargo of EVs have been characterised in many parasites, little is known about the molecules released by the rumen fluke, Calicophoron daubneyi – an emerging infection of livestock in Western Europe. Here we used a mass spectrometry-based proteomics approach to characterise the secretome of the infective newly-excysted juvenile (NEJ) stage and adult flukes. We also profile the proteome of two sub-populations of EVs (termed 15K and 120K EVs) released by the adult flukes.
HostingRepository	PRIDE
AnnounceDate	2021-02-16
AnnouncementXML	Submission_2021-02-16_02:47:29.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014550
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Mark Robinson
SpeciesList	scientific name: Calicophoron daubneyi; NCBI TaxID: 300641;
ModificationList	Deamidated; Oxidation; Dioxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-10 02:38:01	ID requested
⏵ 1	2021-02-16 02:47:30	announced

Huson KM, Atcheson E, Oliver NAM, Best P, Barley JP, Hanna REB, McNeilly TN, Fang Y, Haldenby S, Paterson S, Robinson MW, Transcriptome and Secretome Analysis of Intra-Mammalian Life-Stages of Calicophoron daubneyi Reveals Adaptation to a Unique Host Environment. Mol Cell Proteomics, 20():100055(2021) [pubmed]

submitter keyword: Calicophoron daubneyi, exosome, extracellular vesicle, secretome

Mark W. Robinson
contact affiliation	Institute for Global Food Security School of Biological Sciences Queen's University Belfast 19 Chlorine Gardens Belfast, BT9 5DL
contact email	mark.robinson@qub.ac.uk
lab head
Mark Robinson
contact affiliation	Queens University Belfast
contact email	mark.robinson@qub.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014550
     1. Label: PRIDE project
     2. Name: The secretome of the rumen fluke, Calicophoron daubneyi