PXD014547

PXD014547 is an original dataset announced via ProteomeXchange.

Title	PepSAVI-MS reveals proline-rich antimicrobial peptide in Amaranthus tricolor
Description	PepSAVI-MS, a mass spectrometry-based peptidomics pipeline, was implemented for antimicrobial peptide (AMP) discovery in the medicinal plant Amaranthus tricolor. This investigation revealed a novel 1.7 kDa AMP, deemed Atr-AMP1. Initial efforts to determine the sequence of Atr-AMP1 utilized chemical derivatization and enzymatic digestion to provide information about specific residues and post-translational modifications. EThcD (electron-transfer/higher-energy collision dissociation) produced extensive backbone fragmentation and facilitated de novo sequencing, the results of which were consistent with orthogonal characterization experiments. Additionally, multistage HCD (higher-energy collisional dissociation) facilitated discrimination between isobaric leucine and isoleucine. These results revealed a positively-charged proline-rich peptide present in a heterogeneous population of multiple peptidoforms, possessing several post-translational modifications including a disulfide bond, methionine oxidation, and proline hydroxylation.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_14:14:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tessa Bartges
SpeciesList	scientific name: Amaranthus tricolor; NCBI TaxID: 29722;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-10 01:44:32	ID requested
⏵ 1	2020-05-26 14:14:09	announced

Moyer TB, Heil LR, Kirkpatrick CL, Goldfarb D, Lefever WA, Parsley NC, Wommack AJ, Hicks LM, . J Nat Prod, 82(10):2744-2753(2019) [pubmed]

submitter keyword: Antimicrobial peptides, PepSAVI-MS, Amaranthus tricolor, E. coli, hydroxyproline, mass spectrometry, peptidomics

Leslie Hicks
contact affiliation	Department of Chemistry, Univeristy of North Carolina at Chapel Hill
contact email	lmhicks@email.unc.edu
lab head
Tessa Bartges
contact affiliation	UNC-CHAPEL HILL
contact email	teb12@live.unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014547
     1. Label: PRIDE project
     2. Name: PepSAVI-MS reveals proline-rich antimicrobial peptide in Amaranthus tricolor