PXD014545

PXD014545 is an original dataset announced via ProteomeXchange.

Title	mTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification
Description	Stable isotope labeling of peptides is the basis for numerous mass spectrometry-based quantification strategies. Isobaric tagging and metabolic labeling, namely TMT and SILAC, are among the most widely used techniques for relative protein quantification. Here we report an alternative, precursor-based quantification method using non-isobaric TMT variants: TMT0 (TMTzero) and shTMT (super-heavy TMT). We term this strategy mTMT (mass difference tandem mass tagging), as these TMT variants differ by 11 mass units; yet, peptides labeled with these reagents co-elute, analogous to SILAC-labeled sample analysis. As proof-of-concept, we profiled the proteomes of two cell lines that are frequently used in neuroscience studies, SH-SY5Y and SVGp12, using mTMT and standard SILAC-labeling approaches. We show similar numbers of quantified proteins and peptides using each method with highly correlated fold changes between workflows. We conclude that mTMT is a suitable alternative for precursor-based protein quantification.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:04:03.421.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joao Paulo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-10 01:43:22	ID requested
1	2019-09-16 00:59:45	announced
⏵ 2	2024-10-22 04:04:08	announced	2024-10-22: Updated project metadata.

10.1021/acs.analchem.9b03162;

Paulo JA, Gygi SP, mTMT: An Alternative, Nonisobaric, Tandem Mass Tag Allowing for Precursor-Based Quantification. Anal Chem, 91(19):12167-12172(2019) [pubmed]

submitter keyword: SILAC,TMT, isobaric, SH-SY5Y, SVGp12, metabolic

Joao A. Paulo
contact affiliation	Joao Paulo Cell Biology Harvard Medical School USA
contact email	joao_paulo@post.harvard.edu
lab head
Joao Paulo
contact affiliation	Harvard Medical School
contact email	joao_paulo@post.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014545

PRIDE project URI

• PRIDE
  1. PXD014545
     1. Label: PRIDE project
     2. Name: mTMT: An alternative, non-isobaric, tandem mass tag allowing for precursor-based quantification