PXD014535

PXD014535 is an original dataset announced via ProteomeXchange.

Title	Characterization of the Response of A549 Cells to Aspergillus fumigatus and Pseudomonas aeruginosa: A Proteomic Perspective
Description	The following describes additional preliminary data to that outlined in Project PXD014022 and specifically describes the 12-hour co-exposure and P. aeruginosa 12 hour exposure preliminary experiments. Aspergillus fumigatus and Pseudomonas aeruginosa are the most prevalent fungal and bacterial pathogens associated with cystic fibrosis-related infections, respectively. P. aeruginosa eventually predominates as the primary pathogen, though it is unknown why this is the case. Label-free quantitative (LFQ) proteomics was employed to characterize the cellular response to co-exposure of A. fumigatus and P. aeruginosa using the type II alveolar epithelial cell line, A549, as a model of the alveolar surface. Proteomic data revealed that P. aeruginosa replication increased exponentially when co-cultured with A. fumigatus. Comparative analysis using LFQ proteomics revealed similarities and distinct differences in the response of A549 cells to A. fumigatus, or P. aeruginosa and sequential exposure to both pathogens. In total, 2264 proteins were identified. Analysis of statistically significant (p<0.05; ±1.5 fold change) differentially abundant (SSDA) proteins, revealed an increase in the relative abundance of proteins associated with biological processes common to all pathogen-exposed groups.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:03:56.334.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Carolan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-09 06:08:13	ID requested
1	2019-11-11 03:01:08	announced
⏵ 2	2024-10-22 04:04:04	announced	2024-10-22: Updated project metadata.

Margalit A, Kavanagh K, Carolan JC, . J Proteome Res, 19(1):279-291(2020) [pubmed]

10.1021/acs.jproteome.9b00520;

submitter keyword: Aspergillus

Cellular response

Cystic fibrosis

Fungal infection

Pseudomonas

James Carolan
contact affiliation	Department of Biology, Maynooth University,Maynooth, Co Kildare, Ireland
contact email	james.c.carolan@gmail.com
lab head
James Carolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014535

PRIDE project URI

• PRIDE
  1. PXD014535
     1. Label: PRIDE project
     2. Name: Characterization of the Response of A549 Cells to Aspergillus fumigatus and Pseudomonas aeruginosa: A Proteomic Perspective