PXD014533

PXD014533 is an original dataset announced via ProteomeXchange.

Title	Identification of nutrient dependend PIK3C2B regulators
Description	To identify factors responsible for the differential localization and activity of PIK3C2Bin the presence or absence of serum mitogens we took a quantitative functional proteomics approach based on stable isotope labeling of amino acids in cell culture (SILAC). Genome-engineered HEK293T cells endogenously expressing eGFP-PI3KC15were cultured in serum-containing or serum-deprived media containing heavy or light amino acids, and subjected to affinity capture using a GFP trap matrix and LC-MS/MS
HostingRepository	PRIDE
AnnounceDate	2019-10-02
AnnouncementXML	Submission_2019-10-02_04:10:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Wallroth
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-09 03:33:06	ID requested
⏵ 1	2019-10-02 04:10:36	announced

Wallroth A, Koch PA, Marat AL, Krause E, Haucke V, Protein kinase N controls a lysosomal lipid switch to facilitate nutrient signalling via mTORC1. Nat Cell Biol, 21(9):1093-1101(2019) [pubmed]

curator keyword: Biological

submitter keyword: PIK3C2B

Volker Haucke
contact affiliation	Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), 13125 Berlin, Germany. 2Freie Universität Berlin, Faculty of Biology, Chemistry, Pharmacy, 14195 Berlin, Germany.
contact email	haucke@fmp-berlin.de
lab head
Alexander Wallroth
contact affiliation	FMP
contact email	wallroth@fmp-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014533

PRIDE project URI

• PRIDE
  1. PXD014533
     1. Label: PRIDE project
     2. Name: Identification of nutrient dependend PIK3C2B regulators