PXD014515

PXD014515 is an original dataset announced via ProteomeXchange.

Title	Blood contamination in CSF and its impact on quantitative analysis of alpha-synuclein
Description	Analysis of cerebrospinal fluid (CSF) is important for diagnosis of neurological diseases. Specific proteins, which are released from the CNS can be quantitatively monitored. Especially, in the field of neurodegenerative diseases abnormal protein abundance is an important biomarker. However, the quality of the CSF sample is a key factor for the analytic outcome. In this study we evaluated the effect of blood contamination in CSF with respect to protein biomarker identification. We compared three distinct measures: semiquantitative Combur10-Test® strips for urinary hemoglobin, a specific hemoglobin ELISA and bottom-up mass spectrometry (MS) based proteomics for the determination of the general blood contamination level. In parallel, we studied the impact of blood contamination on the detectability of alpha-synuclein (aSyn), a highly abundant protein in blood/erythrocytes and potential biomarker for Parkinson’s disease. Based on our results we identified other markers for blood contamination beyond hemoglobin and defined a grading system for blood levels in CSF samples including a lower limit of tolerable blood contamination for mass spectrometry-based biomarker studies and suggest the Combur10-Test® as a cost-effective test for simple and fast determination of blood contaminations in CSF.
HostingRepository	PRIDE
AnnounceDate	2020-02-18
AnnouncementXML	Submission_2020-02-17_21:43:19.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014515
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Katalin Barkovits
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-08 03:16:49	ID requested
⏵ 1	2020-02-17 21:43:21	announced

Barkovits K, Kruse N, Linden A, T, ö, nges L, Pfeiffer K, Mollenhauer B, Marcus K, Blood Contamination in CSF and Its Impact on Quantitative Analysis of Alpha-Synuclein. Cells, 9(2):(2020) [pubmed]

curator keyword: Technical, Biological

submitter keyword: cerebrospinal fluid, blood contamination, bottom-up mass spectrometry, ELISA, urine strip, alpha-synuclein, quantification

Katrin Marcus
contact affiliation	Ruhr-Universität Bochum, Medizinisches Proteom-Center, Germany
contact email	katrin.marcus@rub.de
lab head
Katalin Barkovits
contact affiliation	Ruhr University Bochum
contact email	katalin.barkovits@rub.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014515
     1. Label: PRIDE project
     2. Name: Blood contamination in CSF and its impact on quantitative analysis of alpha-synuclein