PXD014508 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The liver proteome in a mouse model for Ascaris suum resistance and susceptibility – evidence for an altered innate immune response |
Description | Ascariasis is a neglected tropical disease that affects 800 million people worldwide. Whereas most people only experience light worm burden, some people experience heavy worm burdens even after several rounds of chemotherapy, a phenomenon known as predisposition. Such heavy infections are associated with more severe symptoms and increased chronic morbidity. In order to investigate potential mechanisms that may explain the observed predisposition, we infected mice with the porcine ascarid A. suum using an established mouse model with two different mouse strains, C57BL/6J and CBA/Ca, as a model for heavy and light infection. At day 7 post infection we investigated the liver proteome, using shotgun mass spectrometry, of both infected and control mice of each strain. We identified intrinsic differences, between the two mouse strains, in both oxidative phosphorylation proteins and proteins involved in the retinol metabolism. Additionally, we found differences between the two mouse strains in activation of the complement system, where the resistant mouse strain has higher protein abundances for lectin pathway proteins and the susceptible mouse strain has higher protein abundances for complement inhibiting proteins. The resistant mouse strain had a higher abundance of proteins involved in the activation of the complement cascade via the lectin pathway. In contrast, the susceptible strain demonstrated a higher abundance of proteins involved in arresting the complement pathway. In conclusion, we observed clear differences between the two mouse strains both intrinsically and under infection. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:03:42.510.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | James Carolan |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-08 01:11:53 | ID requested | |
1 | 2019-11-11 17:14:18 | announced | |
⏵ 2 | 2024-10-22 04:03:50 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1186/s13071-019-3655-9; |
Deslyper G, Holland CV, Colgan TJ, Carolan JC, The liver proteome in a mouse model for Ascaris suum resistance and susceptibility: evidence for an altered innate immune response. Parasit Vectors, 12(1):402(2019) [pubmed] |
Keyword List
submitter keyword: shotgun mass spectrometry, innate immune response, liver, mitochondria, proteome,Ascaris |
Contact List
James Carolan |
contact affiliation | Department of Biology, Maynooth University |
contact email | james.c.carolan@gmail.com |
lab head | |
James Carolan |
contact affiliation | Biology |
contact email | james.carolan@nuim.ie |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014508
- Label: PRIDE project
- Name: The liver proteome in a mouse model for Ascaris suum resistance and susceptibility – evidence for an altered innate immune response