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PXD014508

PXD014508 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe liver proteome in a mouse model for Ascaris suum resistance and susceptibility – evidence for an altered innate immune response
DescriptionAscariasis is a neglected tropical disease that affects 800 million people worldwide. Whereas most people only experience light worm burden, some people experience heavy worm burdens even after several rounds of chemotherapy, a phenomenon known as predisposition. Such heavy infections are associated with more severe symptoms and increased chronic morbidity. In order to investigate potential mechanisms that may explain the observed predisposition, we infected mice with the porcine ascarid A. suum using an established mouse model with two different mouse strains, C57BL/6J and CBA/Ca, as a model for heavy and light infection. At day 7 post infection we investigated the liver proteome, using shotgun mass spectrometry, of both infected and control mice of each strain. We identified intrinsic differences, between the two mouse strains, in both oxidative phosphorylation proteins and proteins involved in the retinol metabolism. Additionally, we found differences between the two mouse strains in activation of the complement system, where the resistant mouse strain has higher protein abundances for lectin pathway proteins and the susceptible mouse strain has higher protein abundances for complement inhibiting proteins. The resistant mouse strain had a higher abundance of proteins involved in the activation of the complement cascade via the lectin pathway. In contrast, the susceptible strain demonstrated a higher abundance of proteins involved in arresting the complement pathway. In conclusion, we observed clear differences between the two mouse strains both intrinsically and under infection.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:03:42.510.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJames Carolan
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-08 01:11:53ID requested
12019-11-11 17:14:18announced
22024-10-22 04:03:50announced2024-10-22: Updated project metadata.
Publication List
10.1186/s13071-019-3655-9;
Deslyper G, Holland CV, Colgan TJ, Carolan JC, The liver proteome in a mouse model for Ascaris suum resistance and susceptibility: evidence for an altered innate immune response. Parasit Vectors, 12(1):402(2019) [pubmed]
Keyword List
submitter keyword: shotgun mass spectrometry, innate immune response, liver, mitochondria, proteome,Ascaris
Contact List
James Carolan
contact affiliationDepartment of Biology, Maynooth University
contact emailjames.c.carolan@gmail.com
lab head
James Carolan
contact affiliationBiology
contact emailjames.carolan@nuim.ie
dataset submitter
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Dataset FTP location
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