PXD014508

PXD014508 is an original dataset announced via ProteomeXchange.

Title	The liver proteome in a mouse model for Ascaris suum resistance and susceptibility – evidence for an altered innate immune response
Description	Ascariasis is a neglected tropical disease that affects 800 million people worldwide. Whereas most people only experience light worm burden, some people experience heavy worm burdens even after several rounds of chemotherapy, a phenomenon known as predisposition. Such heavy infections are associated with more severe symptoms and increased chronic morbidity. In order to investigate potential mechanisms that may explain the observed predisposition, we infected mice with the porcine ascarid A. suum using an established mouse model with two different mouse strains, C57BL/6J and CBA/Ca, as a model for heavy and light infection. At day 7 post infection we investigated the liver proteome, using shotgun mass spectrometry, of both infected and control mice of each strain. We identified intrinsic differences, between the two mouse strains, in both oxidative phosphorylation proteins and proteins involved in the retinol metabolism. Additionally, we found differences between the two mouse strains in activation of the complement system, where the resistant mouse strain has higher protein abundances for lectin pathway proteins and the susceptible mouse strain has higher protein abundances for complement inhibiting proteins. The resistant mouse strain had a higher abundance of proteins involved in the activation of the complement cascade via the lectin pathway. In contrast, the susceptible strain demonstrated a higher abundance of proteins involved in arresting the complement pathway. In conclusion, we observed clear differences between the two mouse strains both intrinsically and under infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:03:42.510.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	James Carolan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-08 01:11:53	ID requested
1	2019-11-11 17:14:18	announced
⏵ 2	2024-10-22 04:03:50	announced	2024-10-22: Updated project metadata.

10.1186/s13071-019-3655-9;

Deslyper G, Holland CV, Colgan TJ, Carolan JC, The liver proteome in a mouse model for Ascaris suum resistance and susceptibility: evidence for an altered innate immune response. Parasit Vectors, 12(1):402(2019) [pubmed]

submitter keyword: shotgun mass spectrometry, innate immune response, liver, mitochondria, proteome,Ascaris

James Carolan
contact affiliation	Department of Biology, Maynooth University
contact email	james.c.carolan@gmail.com
lab head
James Carolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014508
     1. Label: PRIDE project
     2. Name: The liver proteome in a mouse model for Ascaris suum resistance and susceptibility – evidence for an altered innate immune response