PXD014504

PXD014504 is an original dataset announced via ProteomeXchange.

Title	Glycosaminoglycan Domain Mapping of cellular chondroitin/dermatan sulfates
Description	Here we report a simple and versatile approach for domain mapping of complex mixtures of glycosaminoglycans (GAGs), GAGDoMa. The approach is based on orthogonal enzymatic depolymerization of the GAGs, generating internal oligosaccharide, non-reducing end, and linkage region GAG domains, nanoflow reversed-phase dibutylamine ion-pairing chromatography and negative mode higher-energy collision dissociation (HCD) MS/MS. GAGDoMa provides a detailed insight into the GAGome, and will be an important tool for the understanding of GAGs in cellular physiology and pathology.
HostingRepository	PRIDE
AnnounceDate	2020-02-18
AnnouncementXML	Submission_2020-05-18_05:57:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Egor Vorontsov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-05 03:11:03	ID requested
1	2020-02-18 00:40:24	announced
⏵ 2	2020-05-18 05:58:03	announced	2020-05-18: Updated publication reference for PubMed record(s): 32103093.

Persson A, Vorontsov E, Larson G, Nilsson J, Glycosaminoglycan Domain Mapping of Cellular Chondroitin/Dermatan Sulfates. Sci Rep, 10(1):3506(2020) [pubmed]

submitter keyword: CHondroitin sulfate, dermatan sulfate, glycosaminoglycans, GAGs, glycomics, higher-energy collision dissociation, HCD, Orbitrap Elite, negative ionisation mode, ion-pairing reversed-phase chromatography

Jonas Nilsson
contact affiliation	Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine, University of Gothenburg, Gothenburg, Sweden
contact email	jonas.nilsson@clinchem.gu.se
lab head
Egor Vorontsov
contact affiliation	Proteomics Core Facility, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden
contact email	yegor.msu@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD014504

PRIDE project URI

• PRIDE
  1. PXD014504
     1. Label: PRIDE project
     2. Name: Glycosaminoglycan Domain Mapping of cellular chondroitin/dermatan sulfates