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PXD014501

PXD014501 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMolecular basis of b-adrenergic regulation of cardiac calcium current
DescriptionIncreased cardiac contractility during fight-or-flight response is caused by b-adrenergic augmentation of CaV1.2 channels. It is assumed that this iconic regulation involves phosphorylation of CaV1.2 a1/b-subunits. In transgenic murine hearts expressing fully PKA phosphorylation-deficient mutant a1C/b, this regulation persists, however, suggesting involvement of extra-channel factors. We here show that PKA up-regulates cardiac CaV1.2 channels by phosphorylating the small G-protein Rad, relieving constitutive inhibition of CaV1.2. Peroxidase-catalyzed labeling in mice hearts expressing ascorbate peroxidase conjugated-a1C or b2b with multiplexed quantitative proteomics allowed tracking of thousands of proteins in proximity of CaV1.2.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:08:41.032.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMarian Kalocsay
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListiodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-05 01:11:55ID requested
12019-11-19 08:33:14announced
22020-01-29 06:26:51announced2020-01-29: Updated publication reference for PubMed record(s): 31969708.
32024-10-22 04:08:45announced2024-10-22: Updated project metadata.
Publication List
Liu G, Papa A, Katchman AN, Zakharov SI, Roybal D, Hennessey JA, Kushner J, Yang L, Chen BX, Kushnir A, Dangas K, Gygi SP, Pitt GS, Colecraft HM, Ben-Johny M, Kalocsay M, Marx SO, 1.2 stimulation revealed by proximity proteomics. Nature, 577(7792):695-700(2020) [pubmed]
10.1038/s41586-020-1947-z;
Keyword List
submitter keyword: isobaric labeling, isoproterenol, TMT, alkaline reversed phase peptide fractionation, non-transgenic,Cardiomyocytes, quantitative mass spectrometry, SPS MS3, b-adrenergic regulation
Contact List
Marian Kalocsay
contact affiliationDepartment of Systems Biology, Laboratory of Systems Pharmacology, Harvard Medical School, Boston MA, 02115
contact emailmarian_kalocsay@hms.harvard.edu
lab head
Marian Kalocsay
contact affiliationThe University of Texas MD Anderson Cancer Center
contact emailmarian_kalocsay@hms.harvard.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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