PXD014500

PXD014500 is an original dataset announced via ProteomeXchange.

Title	Molecular basis of b-adrenergic regulation of cardiac calcium current
Description	Increased cardiac contractility during fight-or-flight response is caused by b-adrenergic augmentation of CaV1.2 channels. It is assumed that this iconic regulation involves phosphorylation of CaV1.2 a1/b-subunits. In transgenic murine hearts expressing fully PKA phosphorylation-deficient mutant a1C/b, this regulation persists, however, suggesting involvement of extra-channel factors. We here show that PKA up-regulates cardiac CaV1.2 channels by phosphorylating the small G-protein Rad, relieving constitutive inhibition of CaV1.2. Peroxidase-catalyzed labeling in mice hearts expressing ascorbate peroxidase conjugated-a1C or b2b with multiplexed quantitative proteomics allowed tracking of thousands of proteins in proximity of CaV1.2.
HostingRepository	PRIDE
AnnounceDate	2019-11-19
AnnouncementXML	Submission_2020-01-29_06:26:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marian Kalocsay
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-05 01:11:29	ID requested
1	2019-11-19 04:41:25	announced
⏵ 2	2020-01-29 06:26:27	announced	2020-01-29: Updated publication reference for PubMed record(s): 31969708.

Liu G, Papa A, Katchman AN, Zakharov SI, Roybal D, Hennessey JA, Kushner J, Yang L, Chen BX, Kushnir A, Dangas K, Gygi SP, Pitt GS, Colecraft HM, Ben-Johny M, Kalocsay M, Marx SO, 1.2 stimulation revealed by proximity proteomics. Nature, 577(7792):695-700(2020) [pubmed]

submitter keyword: Cav1.2, cardiac calcium channel, b-adrenergic regulation, APEX2, peroxidase catalyzed, proximity labeling, biotin-tyramide, H2O2, denaturing purification, streptavidin, on-bead digest, isobaric labeling, TMT, alkaline reversed phase peptide fractionation, quantitative mass spectrometry, SPS MS3, biotin-phenol, APEX, isoproterenol

Marian Kalocsay
contact affiliation	Department of Systems Biology, Laboratory for Systems Pharmacology, Harvard Medical School, Boston MA, USA
contact email	marian_kalocsay@hms.harvard.edu
lab head
Marian Kalocsay
contact affiliation	Harvard Medical School
contact email	marian_kalocsay@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD014500

PRIDE project URI

• PRIDE
  1. PXD014500
     1. Label: PRIDE project
     2. Name: Molecular basis of b-adrenergic regulation of cardiac calcium current