PXD014487

PXD014487 is an original dataset announced via ProteomeXchange.

Title	Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamics
Description	Extracellular vesicles are emerging key actors in adipocyte communication. Notably, small extracellular vesicles shed by adipocytes promote melanoma aggressiveness through fatty acid oxidation, with a heightened effect in obesity. However, the vesicular actors and cellular processes involved remain largely unknown. Here, we elucidate the mechanisms linking adipocyte extracellular vesicles to metabolic remodeling and cell migration. Using an adapted SILAC technique, we show that adipocyte vesicles stimulate melanoma fatty acid oxidation by providing both enzymes and substrates. In obesity, the heightened effect of extracellular vesicles depends on increased transport of fatty acids, not fatty acid oxidation related enzymes as shown by classical LC-MS/MS analysis. These fatty acids, stored within lipid droplets in cancer cells, drive fatty acid oxidation after release through lipophagy. This increase in mitochondrial activity redistributes mitochondria to membrane protrusions of migrating cells, which is necessary to increase cell migration in the presence of adipocyte vesicles. Our results provide key insights into the role of extracellular vesicles in the metabolic cooperation that takes place between adipocytes and tumors with particular relevance in obesity.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:06:36.280.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manuelle Ducoux-Petit
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-04 08:15:09	ID requested
1	2020-01-09 09:46:20	announced
2	2020-01-17 06:30:09	announced	2020-01-17: Updated publication reference for PubMed record(s): 31919869.
3	2020-02-17 02:25:19	announced	2020-01-17: Updated publication reference for PubMed record(s): 31919869. 2020-02-17: Updated publication reference for PubMed record(s): 31919869.
⏵ 4	2024-10-22 04:06:38	announced	2024-10-22: Updated project metadata.

Clement E, Lazar I, Attan, é C, Carri, é L, Dauvillier S, Ducoux-Petit M, Esteve D, Menneteau T, Moutahir M, Le Gonidec S, Dalle S, Valet P, Burlet-Schiltz O, Muller C, Nieto L, Adipocyte extracellular vesicles carry enzymes and fatty acids that stimulate mitochondrial metabolism and remodeling in tumor cells. EMBO J, 39(3):e102525(2020) [pubmed]

10.15252/embj.2019102525;

submitter keyword: exosome / fatty acid oxidation / lipophagy / melanoma / obesity / LC-MSMS

Odile Burlet-Schiltz
contact affiliation	Proteomics and Mass Spectrometry of Biomolecules Institute of Pharmacology and Structural Biology (IPBS), Université de Toulouse, CNRS, UPS, 31077 Toulouse, France
contact email	Odile.Schiltz@ipbs.fr
lab head
Manuelle Ducoux-Petit
contact affiliation	CNRS
contact email	manuelle.ducoux@ipbs.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014487

PRIDE project URI

• PRIDE
  1. PXD014487
     1. Label: PRIDE project
     2. Name: Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamics