PXD014479

PXD014479 is an original dataset announced via ProteomeXchange.

Title	N-terminomics/TAILS profiling of proteases and their substrates in ulcerative colitis
Description	Dysregulated protease activity is often implicated in the initiation of inflammation and immune cell recruitment in gastrointestinal inflammatory diseases. Using N-terminomics/TAILS (terminal amine isotopic labeling of substrates), we compared proteases, along with their substrates and inhibitors, between colonic mucosal biopsies of healthy patients and those with ulcerative colitis (UC). Among the 1,642 N-termini enriched using TAILS, increased endogenous processing of proteins was identified in UC compared to healthy patients. Changes in the reactome pathways for proteins associated with metabolism, adherens junction proteins (E-cadherin, liver-intestinal cadherin, catenin alpha-1 and catenin delta-1) and neutrophil degranulation were identified between the two groups. Increased neutrophil infiltration and distinct proteases observed in ulcerative colitis may result in extensive break down, altered processing or increased remodeling of adherens junctions and other cellular functions. Analysis of the proteolytic preferred cleavage sites indicated that the majority of proteolytic activity and processing comes from host proteases, but that key microbial proteases may also play a role in maintaining homeostasis. Thus, the identification of distinct proteases and processing of their substrates improves the understanding of dysregulated proteolysis in normal intestinal physiology and ulcerative colitis.
HostingRepository	PRIDE
AnnounceDate	2019-09-08
AnnouncementXML	Submission_2019-09-09_02:43:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antoine Dufour
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; dimethylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-04 02:23:24	ID requested
⏵ 1	2019-09-09 02:43:51	announced

Gordon MH, Anowai A, Young D, Das N, Campden RI, Sekhon H, Myers Z, Mainoli B, Chopra S, Thuy-Boun PS, Kizhakkedathu J, Bindra G, Jijon HB, Heitman S, Yates R, Wolan DW, Edgington-Mitchell LE, MacNaughton WK, Dufour A, N-Terminomics/TAILS Profiling of Proteases and Their Substrates in Ulcerative Colitis. ACS Chem Biol, 14(11):2471-2483(2019) [pubmed]

submitter keyword: protease, TAILS, ulcerative colitis, biopsies

Antoine Dufour
contact affiliation	Physiology and Pharmacology McCaig Institute Southern Alberta Mass Spectrometry Core facility University of Calgary
contact email	antoine.dufour@ucalgary.ca
lab head
Antoine Dufour
contact affiliation	University of Calgary Department of Physiology and Pharmacology
contact email	antoine.dufour@ucalgary.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/09/PXD014479

PRIDE project URI

• PRIDE
  1. PXD014479
     1. Label: PRIDE project
     2. Name: N-terminomics/TAILS profiling of proteases and their substrates in ulcerative colitis