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PXD014478

PXD014478 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus
DescriptionPurpose: Staphylococcus aureus is a highly successful human pathogen responsible for wide range of infections. In this study, we provide insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin susceptible and methicillin resistant Staphylococcus aureus (MSSA; MRSA) recovered from non-healthcare environments. Experiment design: Three environmental MSSA and three environmental MRSA were selected for proteomic profiling using iTRAQ MS/MS. Gene Ontology (GO) Annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Annotation were applied to interpret the functions of the proteins detected. Results: 792 proteins were identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA revealed that 8 of out 792 proteins were up-regulated and 156 down-regulated. Differentially abundant proteins were predominantly involved in catalytic and binding activity. Among 164 proteins that had differences in abundance, 29 proteins were involved in pathogenesis, antimicrobial activities,stress response, mismatch repair and cell wall synthesis. Twenty-two proteins associated with pathogenicity, including spa, sbi, clfA and dlt were up-regulated in MRSA. Moreover, the up-regulated pathogenic protein entC2 in MSSA was determined to be a super antigen potentially capable of triggering toxic shock syndrome in the host. Conclusions: Enhanced pathogenicity, antimicrobial activity and stress response were observed in MRSA compared to MSSA.
HostingRepositoryPRIDE
AnnounceDate2020-01-09
AnnouncementXMLSubmission_2020-01-09_03:02:13.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterZhen Xu
SpeciesList scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-04 02:22:50ID requested
12020-01-09 03:02:15announced
Publication List
Xu Z, Chen J, Vougas K, Shah A, Shah H, Misra R, Mkrtchyan HV, Comparative Proteomic Profiling of Methicillin-Susceptible and Resistant Staphylococcus aureus. Proteomics, 20(2):e1900221(2020) [pubmed]
Keyword List
submitter keyword: Comparative proteomics, MRSA, MSSA
Contact List
Hermine V Mkrtchyan
contact affiliationSchool of Health, Sport and Biosciences, University of East London
contact emailH.Mkrtchyan@uel.ac.uk
lab head
Zhen Xu
contact affiliationDr
contact emailxuzhen@tmu.edu.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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