PXD014478 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus |
Description | Purpose: Staphylococcus aureus is a highly successful human pathogen responsible for wide range of infections. In this study, we provide insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin susceptible and methicillin resistant Staphylococcus aureus (MSSA; MRSA) recovered from non-healthcare environments. Experiment design: Three environmental MSSA and three environmental MRSA were selected for proteomic profiling using iTRAQ MS/MS. Gene Ontology (GO) Annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Annotation were applied to interpret the functions of the proteins detected. Results: 792 proteins were identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA revealed that 8 of out 792 proteins were up-regulated and 156 down-regulated. Differentially abundant proteins were predominantly involved in catalytic and binding activity. Among 164 proteins that had differences in abundance, 29 proteins were involved in pathogenesis, antimicrobial activities,stress response, mismatch repair and cell wall synthesis. Twenty-two proteins associated with pathogenicity, including spa, sbi, clfA and dlt were up-regulated in MRSA. Moreover, the up-regulated pathogenic protein entC2 in MSSA was determined to be a super antigen potentially capable of triggering toxic shock syndrome in the host. Conclusions: Enhanced pathogenicity, antimicrobial activity and stress response were observed in MRSA compared to MSSA. |
HostingRepository | PRIDE |
AnnounceDate | 2020-01-09 |
AnnouncementXML | Submission_2020-01-09_03:02:13.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Zhen Xu |
SpeciesList | scientific name: Staphylococcus aureus; NCBI TaxID: 1280; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-07-04 02:22:50 | ID requested | |
⏵ 1 | 2020-01-09 03:02:15 | announced | |
Publication List
Xu Z, Chen J, Vougas K, Shah A, Shah H, Misra R, Mkrtchyan HV, Comparative Proteomic Profiling of Methicillin-Susceptible and Resistant Staphylococcus aureus. Proteomics, 20(2):e1900221(2020) [pubmed] |
Keyword List
submitter keyword: Comparative proteomics, MRSA, MSSA |
Contact List
Hermine V Mkrtchyan |
contact affiliation | School of Health, Sport and Biosciences, University of East London |
contact email | H.Mkrtchyan@uel.ac.uk |
lab head | |
Zhen Xu |
contact affiliation | Dr |
contact email | xuzhen@tmu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014478
- Label: PRIDE project
- Name: Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus