PXD014478

PXD014478 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus
Description	Purpose: Staphylococcus aureus is a highly successful human pathogen responsible for wide range of infections. In this study, we provide insights into the virulence, pathogenicity, and antimicrobial resistance determinants of methicillin susceptible and methicillin resistant Staphylococcus aureus (MSSA; MRSA) recovered from non-healthcare environments. Experiment design: Three environmental MSSA and three environmental MRSA were selected for proteomic profiling using iTRAQ MS/MS. Gene Ontology (GO) Annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Annotation were applied to interpret the functions of the proteins detected. Results: 792 proteins were identified in MSSA and MRSA. Comparative analysis of MRSA and MSSA revealed that 8 of out 792 proteins were up-regulated and 156 down-regulated. Differentially abundant proteins were predominantly involved in catalytic and binding activity. Among 164 proteins that had differences in abundance, 29 proteins were involved in pathogenesis, antimicrobial activities，stress response, mismatch repair and cell wall synthesis. Twenty-two proteins associated with pathogenicity, including spa, sbi, clfA and dlt were up-regulated in MRSA. Moreover, the up-regulated pathogenic protein entC2 in MSSA was determined to be a super antigen potentially capable of triggering toxic shock syndrome in the host. Conclusions: Enhanced pathogenicity, antimicrobial activity and stress response were observed in MRSA compared to MSSA.
HostingRepository	PRIDE
AnnounceDate	2020-01-09
AnnouncementXML	Submission_2020-01-09_03:02:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhen Xu
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-04 02:22:50	ID requested
⏵ 1	2020-01-09 03:02:15	announced

Xu Z, Chen J, Vougas K, Shah A, Shah H, Misra R, Mkrtchyan HV, Comparative Proteomic Profiling of Methicillin-Susceptible and Resistant Staphylococcus aureus. Proteomics, 20(2):e1900221(2020) [pubmed]

submitter keyword: Comparative proteomics, MRSA, MSSA

Hermine V Mkrtchyan
contact affiliation	School of Health, Sport and Biosciences, University of East London
contact email	H.Mkrtchyan@uel.ac.uk
lab head
Zhen Xu
contact affiliation	Dr
contact email	xuzhen@tmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014478

PRIDE project URI

• PRIDE
  1. PXD014478
     1. Label: PRIDE project
     2. Name: Comparative proteomic profiling of methicillin-susceptible and resistant Staphylococcus aureus