PXD014476

PXD014476 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	A reference map of the human proinsulin biosynthetic interaction network
Description	A monoclonal antibody specific for human proinsulin (20G11) was generated and utilized for affinity purification mass spectrometry (AP-MS) for unbiased profiling of proinsulin protein:protein interactions in human islets. Half of each islet prep was treated with the cytokines IL1-beta and IFN-gamma. Immunoprecipitated proinsulin or control IgG immunoprecipitates from human islets were then subjected to state of the art mass spectrometry revealing a rich proinsulin biosynthetic network that is remarkably conserved across a diverse group of donors.
HostingRepository	MassIVE
AnnounceDate	2019-07-03
AnnouncementXML	Submission_2019-07-03_17:48:55.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Rosa Campos
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; TMT6plex
Instrument	LTQ Orbitrap Velos; Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-03 15:25:44	ID requested
⏵ 1	2019-07-03 17:48:56	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Diabetes, Type 2 Diabetes, Interactome, Proinsulin misfolding, Affinity Purification-Mass spectrometry

submitter keyword: Diabetes, Type 2 Diabetes, Interactome, Proinsulin misfolding, Affinity Purification-Mass spectrometry

Contact List

Pamela Itkin-Ansari
contact affiliation	Sanford Burnham Prebys Medical Discovery Institute
contact email	pitkin@sbpdiscovery.org
lab head
Alexandre Rosa Campos
contact affiliation	SBP Medical Discovery Institute
contact email	arosacampos@sbpdiscovery.org
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000084057