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PXD014476 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA reference map of the human proinsulin biosynthetic interaction network
DescriptionA monoclonal antibody specific for human proinsulin (20G11) was generated and utilized for affinity purification mass spectrometry (AP-MS) for unbiased profiling of proinsulin protein:protein interactions in human islets. Half of each islet prep was treated with the cytokines IL1-beta and IFN-gamma. Immunoprecipitated proinsulin or control IgG immunoprecipitates from human islets were then subjected to state of the art mass spectrometry revealing a rich proinsulin biosynthetic network that is remarkably conserved across a diverse group of donors.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlexandre Rosa Campos
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; TMT6plex
InstrumentLTQ Orbitrap Velos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-03 15:25:44ID requested
12019-07-03 17:48:56announced
Publication List
no publication
Keyword List
submitter keyword: Diabetes, Type 2 Diabetes, Interactome, Proinsulin misfolding, Affinity Purification-Mass spectrometry
Contact List
Pamela Itkin-Ansari
contact affiliationSanford Burnham Prebys Medical Discovery Institute
contact emailpitkin@sbpdiscovery.org
lab head
Alexandre Rosa Campos
contact affiliationSBP Medical Discovery Institute
contact emailarosacampos@sbpdiscovery.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
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