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PXD014474

PXD014474 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA targeted strategy for developing proteomic biomarkers: a case study of epithelial ovarian cancer
DescriptionProtein biomarkers for epithelial ovarian cancer are critical for the early detection of the cancer to improve patient prognosis and for the clinical management of the disease to monitor treatment response and to detect recurrences. Unfortunately, the discovery of protein biomarkers is hampered by the limited availability of reliable and sensitive assays needed for the reproducible quantification of proteins in complex biological matrices such as blood plasma. In recent years, targeted mass spectrometry, exemplified by Selected Reaction Monitoring (SRM) has emerged as a method, capable of overcoming this limitation. Here, we present a comprehensive SRM-based strategy for developing plasma-based protein biomarkers for epithelial ovarian cancer and illustrate how the SRM platform, when combined with rigorous experimental design and statistical analysis, can result in detection of predictive analytes. Our biomarker development strategy first involved a discovery-driven proteomic effort to derive potential N-glycoprotein biomarker candidates for plasma-based detection of human ovarian cancer from a genetically engineered mouse model of endometrioid ovarian cancer, which accurately recapitulates the human disease. Next, 65 candidate markers selected from proteins of different abundance in the discovery dataset were reproducibly quantified with SRM assays across a large cohort of over 200 plasma samples from ovarian cancer patients and healthy controls. Finally, these measurements were used to derive a 5-protein signature for distinguishing individuals with epithelial ovarian cancer from healthy controls. The sensitivity of the candidate biomarker signature in combination with CA125 ELISA-based measurements currently used in clinic, exceeded that of CA125 ELISA-based measurements alone. The SRM-based strategy in this study is broadly applicable. It can be used in any study that requires accurate and reproducible quantification of selected proteins in a high-throughput and multiplexed fashion.
HostingRepositoryPanoramaPublic
AnnounceDate2019-08-14
AnnouncementXMLSubmission_2019-08-14_15:16:48.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRuth Huttenhain
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListCarbamidomethyl; Label:13C(6)15N(2); Label:13C(6)15N(4)
InstrumentTSQ Vantage; QTRAP 5500; 4000 QTRAP
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-03 06:48:45ID requested
12019-08-14 15:16:57announced
Publication List
Hüttenhain R, Choi M, Martin de la Fuente L, Oehl K, Chang CV, Zimmermann AK, Malander S, Olsson H, Surinova S, Clough T, Heinzelmann-Schwarz V, Wild PJ, Dinulescu DM, Niméus E, Vitek O, Aebersold R, A Targeted Mass Spectrometry Strategy for Developing Proteomic Biomarkers: A Case Study of Epithelial Ovarian Cancer. Mol Cell Proteomics, 18(9):1836-1850(2019) [pubmed]
Keyword List
submitter keyword: Cancer biomarker, Ovarian cancer, Plasma
Contact List
Ruth Huttenhain
contact affiliationDepartment of Cellular & Molecular Pharmacology, UCSF
contact emailruth.huttenhain@ucsf.edu
lab head
Ruth Huttenhain
contact affiliationDepartment of Cellular & Molecular Pharmacology, UCSF
contact emailruth.huttenhain@ucsf.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI