PXD014472

PXD014472 is an original dataset announced via ProteomeXchange.

Title	Specialization of the Drosophila nuclear export family protein, Nxf3, for piRNA precursor export
Description	The piRNA pathway is a conserved small RNA-based immune system that protects animal germ cell genomes from the harmful effects of transposon mobilisation. In Drosophila ovaries, most piRNAs originate from dual-strand clusters, which generate piRNAs from both genomic strands. Dual-strand clusters use non-canonical transcription mechanisms. Although transcribed by RNA polymerase II, cluster transcripts lack splicing signatures and polyA tails. mRNA processing is important for general mRNA export mediated by Nuclear export factor 1. Although UAP56, a component of the transcription and export complex, has been implicated in piRNA precursor export, it remains unknown how dual-strand cluster transcripts are specifically targeted for piRNA biogenesis by export from the nucleus to cytoplasmic processing centers. Here we report that dual-strand cluster transcript export requires CG13741/Bootlegger and the Drosophila Nuclear export factor family protein, Nxf3. Bootlegger is specifically recruited to piRNA clusters and in turn brings Nxf3. We find that Nxf3 specifically binds to piRNA precursors and is essential for their export to piRNA biogenesis sites, a process that is critical for germline transposon silencing. Our data shed light on how dual-strand clusters bypass canonical mRNA features to be specifically exported via Nxf3, ensuring proper piRNA production
HostingRepository	PRIDE
AnnounceDate	2019-07-29
AnnouncementXML	Submission_2019-08-30_00:42:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emma Kneuss
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-03 03:13:25	ID requested
1	2019-07-29 07:14:05	announced
⏵ 2	2019-08-30 00:42:55	announced	Updated publication reference for PubMed record(s): 31416967.

Kneuss E, Munaf, ò M, Eastwood EL, Deumer US, Preall JB, Hannon GJ, Czech B, nuclear export family protein Nxf3 for piRNA precursor export. Genes Dev, 33(17-18):1208-1220(2019) [pubmed]

submitter keyword: pirna rna export piwi drosophila transposons

Gregory J. Hannon
contact affiliation	Cancer Research UK Cambridge Institute University of Cambridge Li Ka Shing Centre Cambridge CB2 0RE United Kingdom
contact email	Greg.Hannon@cruk.cam.ac.uk
lab head
Emma Kneuss
contact affiliation	Cambridge University
contact email	Emma.Kneuss@cruk.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/07/PXD014472

PRIDE project URI

• PRIDE
  1. PXD014472
     1. Label: PRIDE project
     2. Name: Specialization of the Drosophila nuclear export family protein, Nxf3, for piRNA precursor export