PXD014466

PXD014466 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of alcohol and LPS-treated primary mouse microglia
Description	Microglia, the resident immune cells of the brain, can exhibit a broad range of activation phenotypes, many of which have been implicated in several diseases and disorders of the central nervous system including alcohol use disorders and disorders. By utilizing a method optimized for sensitive and rapid quantitative proteomic analysis of microglia involving suspension trapping (S-Trap), we were able to produce efficient and reproducible protein extraction from low cell yielding primary mouse brains. Using a ~2 h gradient on a 75 cm UPLC column with a modified data dependent acquisition method on a hybrid quadrupole-Orbitrap mass spectrometer (QE Plus), 5,062 total proteins were identified where 4,928 of those proteins were quantifiable by label-free quantitation (with 5 biological replicates). This analysis resulted in the most comprehensive proteomic dataset for ethanol- and LPS-treated primary mouse microglia to date and even expanded upon the well-characterized macrophage/microglia response to LPS treatment. This study also highlights the subtle, yet significant changes ethanol exposure can induce when compared to control. Interestingly, these changes are not consistent with the robust classical activation induced by LPS treatment, but instead align with the emerging theory that ethanol-treated microglia yield an alternative activation response. The contrast to LPS-treated microglia leads us to conclude that ethanol does not elicit a strong inflammatory response but rather might have a general inhibitory effect on multiple pathways such as phagocytosis and cell migration.
HostingRepository	PRIDE
AnnounceDate	2020-04-07
AnnouncementXML	Submission_2020-04-06_23:01:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stanley Stevens
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-03 01:06:30	ID requested
⏵ 1	2020-04-06 23:01:40	announced

Guergues J, Wohlfahrt J, Zhang P, Liu B, Stevens SM, Deep proteome profiling reveals novel pathways associated with pro-inflammatory and alcohol-induced microglial activation phenotypes. J Proteomics, 220():103753(2020) [pubmed]

submitter keyword: Alcohol, ethanol, LPS, neuroinflammation, microglia, glia

Stanley Stevens
contact affiliation	Albany College of Pharmacy and Health Sciences
contact email	stanley.stevens@acphs.edu
lab head
Stanley Stevens
contact affiliation	Albany College of Pharmacy and Health Sciences
contact email	stanley.stevens@acphs.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD014466

PRIDE project URI

• PRIDE
  1. PXD014466
     1. Label: PRIDE project
     2. Name: Proteomic analysis of alcohol and LPS-treated primary mouse microglia