PXD014464

PXD014464 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analyses reveal that energy metabolism and protein biosynthesis reinitiation are responsible for the initiation of bolting induced by high temperature in lettuce (Lactuca sativa L.)
Description	Lettuce (Lactuca sativa L.) as one of the most economically important leaf vegetables, is easy bolting in high temperature condition. Early bolting loses its commodity value and edible value and bring huge losses and waste of resources. However, the initiation and molecular mechanism underlying early bolting induced by high temperature remains largely elusive. To better understand it, a comparative proteomics analysis was conducted on lettuce, in the initiation period of bolting induced by a high temperature (33 oC) and a control temperature (20 oC) using iTRAQ-based proteomics, phenotypic measures, and biological verifications using qRT-PCR. The morphologic and microscopic observation showed that the initiation of bolting was on 8 day after high temperature treatment. Fructose rapidly accumulated after high temperature. In the period of the bolting initiation, of the 3305 identified proteins, a total of 93 proteins were identified with differential abundance, among 38 of these proteins were up-regulated and 55 proteins were down-regulated. About 38% of proteins were involved in metabolic pathway and mainly clustered in energy metabolism and protein synthesis. Furthermore, some proteins involved in sugar synthesis were differentially expressed, which were also related with energy production. This is the first report on the metabolic changes involved in bolting initiation in lettuce. Our study suggested that energy metabolism and ribosomal proteins are pivotal components during bolting initiation. It may bring potential regulatory mechanism for the initiation of early bolting by high temperature and manipulations for breeding in lettuce.
HostingRepository	iProX
AnnounceDate	2019-07-02
AnnouncementXML	Submission_2023-03-01_19:12:50.043.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lifeng Meng
SpeciesList	scientific name: Lactuca sativa var. capitata; NCBI TaxID: 1049369;
ModificationList	iTRAQ8plex reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-02 20:07:34	ID requested
1	2019-07-02 20:15:14	announced
⏵ 2	2023-03-01 19:12:51	announced	2023-03-02: Update publication information.

Hao JH, Su HN, Zhang LL, Liu CJ, Han YY, Qin XX, Fan SX, Quantitative proteomic analyses reveal that energy metabolism and protein biosynthesis reinitiation are responsible for the initiation of bolting induced by high temperature in lettuce (Lactuca sativa L.). BMC Genomics, 22(1):427(2021) [pubmed]

submitter keyword: lettuce, high temperature, bolting, proteome, iTRAQ

Jinghong Hao
contact affiliation	Plant Science and Technology College, Beijing University of Agriculture
contact email	haojinghong2013@126.com
lab head
Lifeng Meng
contact affiliation	Insititute of Apiculture Reseach, Chinese Academy of Agricultural Science
contact email	menglifeng@caas.cn
dataset submitter

iProX dataset URI