PXD014455

PXD014455 is an original dataset announced via ProteomeXchange.

Title	A sulfur-containing volatile emitted by potato-associated bacteria confers protection against late blight through direct anti-oomycete activity
Description	Plant diseases are a major cause for yield losses and new strategies to control them without harming the environment are urgently needed. Plant-associated bacteria contribute to their host’s health in diverse ways, among which the emission of disease-inhibiting volatile organic compounds (VOCs). We have previously reported that VOCs emitted by potato-associated bacteria caused strong in vitro growth inhibition of the late blight causing agent Phytophthora infestans. This work focuses on sulfur-containing VOCs (sVOCs) and demonstrates the high in planta protective potential of S-methyl methane thiosulfonate (MMTS), which fully prevented late blight disease in potato leaves and plantlets without phytotoxic effects, in contrast to other sVOCs. Short exposure times were sufficient to protect plants against infection and MMTS also displayed curative effects. We further show that MMTS’s protective activity was not mediated by the plant immune system but lied in its anti-oomycete activity. Using quantitative proteomics, we determined that different sVOCs caused specific proteome changes in P. infestans, highlighting sulfur metabolism, protein translation and redox balance as main targets. This work brings new perspectives for sustainable protection against the devastating Irish Famine pathogen, while opening new research avenues on the role of sVOCs in the interaction between plants and their microbiome.
HostingRepository	PRIDE
AnnounceDate	2020-01-07
AnnouncementXML	Submission_2020-01-07_09:12:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daniela Zuehlke
SpeciesList	scientific name: Phytophthora infestans; NCBI TaxID: 4787;
ModificationList	monohydroxylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 23:40:24	ID requested
⏵ 1	2020-01-07 09:12:55	announced

Chinchilla D, Bruisson S, Meyer S, Z, ü, hlke D, Hirschfeld C, Joller C, L'Haridon F, M, è, ne-Saffran, é L, Riedel K, Weisskopf L, A sulfur-containing volatile emitted by potato-associated bacteria confers protection against late blight through direct anti-oomycete activity. Sci Rep, 9(1):18778(2019) [pubmed]

curator keyword: Biological

submitter keyword: Phytophthora infestans, sulfuor-containg VOCs, proteomics

Katharina Riedel
contact affiliation	Department of Microbial Physiology and Molecular Biology, Institute of Microbiology, University of Greifswald, Greifswald, Germany
contact email	riedela@uni-greifswald.de
lab head
Daniela Zuehlke
contact affiliation	Ernst Moritz Arndt University Greifswald, Institute of Microbiology
contact email	zuehlke@uni-greifswald.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/01/PXD014455

PRIDE project URI

• PRIDE
  1. PXD014455
     1. Label: PRIDE project
     2. Name: A sulfur-containing volatile emitted by potato-associated bacteria confers protection against late blight through direct anti-oomycete activity