PXD014450

PXD014450 is an original dataset announced via ProteomeXchange.

Title	Proteomic study of Staphylococcus haemolyticus after the host-microbe co-colonisation
Description	In this study, the adhesive and biofilm forming properties of ten clinical and ten commensal S. haemolyticus isolates were examined using standard adhesion and biofilm assays, in addition we selected one clinical S. haemolyticus isolate for bacterial surface shaving. The surface shaving approach was used to identify upregulated S. haemolyticus proteins subsequent to human keratinocyte colonization. Relative quantification of up and downregulated proteins was performed by labelling proteins with tandem mass tags (TMT), prior to Mass Spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:05:29.635.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sahlgrenska Academy Proteomics Core
SpeciesList	scientific name: Staphylococcus haemolyticus; NCBI TaxID: 1283;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 07:10:41	ID requested
1	2020-05-26 14:18:11	announced
⏵ 2	2024-10-22 05:05:30	announced	2024-10-22: Updated project metadata.

Wolden R, Pain M, Karlsson R, Karlsson A, Aarag Fredheim EG, Cavanagh JP, Identification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shaving. BMC Microbiol, 20(1):80(2020) [pubmed]

10.1186/s12866-020-01778-8;

submitter keyword: Tandem mass tag, surface shaving, TMT,Staphylococcus haemolyticus, Q Exactive HF

Roger Karlsson
contact affiliation	Roger Karlsson, PhD, Consultant at Nanoxis Consulting AB, Gothenburg, Sweden
contact email	roger.karlsson@nanoxisconsulting.com
lab head
Sahlgrenska Academy Proteomics Core
contact affiliation	University of Gothenburg
contact email	egor.vorontsov@gu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD014450

PRIDE project URI

• PRIDE
  1. PXD014450
     1. Label: PRIDE project
     2. Name: Proteomic study of Staphylococcus haemolyticus after the host-microbe co-colonisation