PXD014447

PXD014447 is an original dataset announced via ProteomeXchange.

Title	SERRATE interacts with the nuclear exosome targeting complex to degrade primary miRNA precursors in Arabidopsis
Description	0.3g of flowers were ground in liquid nitrogen. The powder was extracted for 30 min [H2] in 1.5 ml of lysis buffer (50mM Tris HCl pH 8.0, 50mM NaCl, 1% Triton x-100 and 1 x cOmplete™ EDTA-free protease inhibitor (Roche)). After removal of cell debris by centrifugation (2 times 10 min, 16000 x g, 4 °C) the cleared supernatants were incubated for 30 min with GFP or FLAG antibodies coupled to magnetic microbeads (μMACS GFP and DYKDDDDK isolation Kits, Miltenyi). Beads were loaded on magnetized MACS separation columns equilibrated with lysis buffer and washed 4 times with 300 µl washing buffer (50mM Tris HCL pH 7.5, 25 or 50 mM NaCl, 0,1 % Triton). Samples were eluted in 50 µl of pre-warmed elution buffer (Milteny). Control IPS were performed in Col-O using either GFP or FLAG antibodies.
HostingRepository	PRIDE
AnnounceDate	2020-04-15
AnnouncementXML	Submission_2020-06-30_04:24:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lauriane Kuhn
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 06:28:48	ID requested
1	2020-04-15 05:13:02	announced
⏵ 2	2020-06-30 04:24:46	announced	2020-06-30: Updated publication reference for PubMed record(s): 32449937.

Bajczyk M, Lange H, Bielewicz D, Szewc L, Bhat SS, Dolata J, Kuhn L, Szweykowska-Kulinska Z, Gagliardi D, Jarmolowski A, SERRATE interacts with the nuclear exosome targeting (NEXT) complex to degrade primary miRNA precursors in Arabidopsis. Nucleic Acids Res, 48(12):6839-6854(2020) [pubmed]

submitter keyword: SERRATE, NEXT, exosome, miRNA biogenesis, pri-miRNAs

Heike Lange
contact affiliation	Institut de Biologie Moléculaire des Plantes, Centre National de la Recherche Scientifique, UPR 2357, Université de Strasbourg, Strasbourg, France.
contact email	heike.lange@ibmp-cnrs.unistra.fr
lab head
Lauriane Kuhn
contact affiliation	CNRS, IBMC, FRC1589
contact email	l.kuhn@ibmc-cnrs.unistra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/04/PXD014447

PRIDE project URI

• PRIDE
  1. PXD014447
     1. Label: PRIDE project
     2. Name: SERRATE interacts with the nuclear exosome targeting complex to degrade primary miRNA precursors in Arabidopsis