PXD014445
PXD014445 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | System-wide identification of enzyme substrates by thermal analysis (SIESTA) |
Description | Despite the immense importance of enzyme-substrate reactions, there is a lack of generic and unbiased tools for identifying the molecular components participating in these reactions on a cellular level. Here we developed a universal method called System-wide Identification of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that enzymatic post-translational modification of substrate proteins changes their thermal stability, and applies the concept of specificity to reveal potential substrates. We have already shown the applicability of SIESTA in substrate discovery for TXNRD1 and PARP10 enzymes. SIESTA successfully identified several known and novel substrate candidates for protein kinase B (AKT1). A number of putative substrates were confirmed by functional assays. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, open new opportunities in investigating the effect of PTMs on protein stability, and facilitate drug discovery. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:17:43.377.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Amir Ata Saei |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2019-07-01 03:42:44 | ID requested | |
1 | 2021-01-11 00:01:53 | announced | |
2 | 2021-04-06 02:11:12 | announced | 2021-04-06: Updated publication reference for PubMed record(s): 33637753. |
⏵ 3 | 2024-10-22 05:17:44 | announced | 2024-10-22: Updated project metadata. |
Publication List
Saei AA, Beusch CM, Sabatier P, Wells JA, Gharibi H, Meng Z, Chernobrovkin A, Rodin S, N, ä, reoja K, Thorsell AG, Karlberg T, Cheng Q, Lundstr, ö, m SL, Gaetani M, V, é, gv, á, ri Á, Arn, é, r ESJ, Sch, ü, ler H, Zubarev RA, System-wide identification and prioritization of enzyme substrates by thermal analysis. Nat Commun, 12(1):1296(2021) [pubmed] |
10.1038/s41467-021-21540-6; |
Keyword List
submitter keyword: proteomics, post-translational modification, target, thermal stability, LC/MS,AKT1, TrxR1, PARP10, ARTD10, RAC-alpha serine threonine protein kinase, TXNRD1 |
Contact List
Roman Zubarev | |
---|---|
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, STOCKHOLM, Sweden |
contact email | roman.zubarev@ki.se |
lab head | |
Amir Ata Saei | |
contact affiliation | Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden |
contact email | amirata.saei.dibavar@ki.se |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD014445 |
PRIDE project URI |
Repository Record List
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