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PXD014445

PXD014445 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSystem-wide identification of enzyme substrates by thermal analysis (SIESTA)
DescriptionDespite the immense importance of enzyme-substrate reactions, there is a lack of generic and unbiased tools for identifying the molecular components participating in these reactions on a cellular level. Here we developed a universal method called System-wide Identification of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that enzymatic post-translational modification of substrate proteins changes their thermal stability, and applies the concept of specificity to reveal potential substrates. We have already shown the applicability of SIESTA in substrate discovery for TXNRD1 and PARP10 enzymes. SIESTA successfully identified several known and novel substrate candidates for protein kinase B (AKT1). A number of putative substrates were confirmed by functional assays. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, open new opportunities in investigating the effect of PTMs on protein stability, and facilitate drug discovery.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:17:43.377.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAmir Ata Saei
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-07-01 03:42:44ID requested
12021-01-11 00:01:53announced
22021-04-06 02:11:12announced2021-04-06: Updated publication reference for PubMed record(s): 33637753.
32024-10-22 05:17:44announced2024-10-22: Updated project metadata.
Publication List
Saei AA, Beusch CM, Sabatier P, Wells JA, Gharibi H, Meng Z, Chernobrovkin A, Rodin S, N, ä, reoja K, Thorsell AG, Karlberg T, Cheng Q, Lundstr, ö, m SL, Gaetani M, V, é, gv, á, ri Á, Arn, é, r ESJ, Sch, ü, ler H, Zubarev RA, System-wide identification and prioritization of enzyme substrates by thermal analysis. Nat Commun, 12(1):1296(2021) [pubmed]
10.1038/s41467-021-21540-6;
Keyword List
submitter keyword: proteomics, post-translational modification, target, thermal stability, LC/MS,AKT1, TrxR1, PARP10, ARTD10, RAC-alpha serine threonine protein kinase, TXNRD1
Contact List
Roman Zubarev
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, STOCKHOLM, Sweden
contact emailroman.zubarev@ki.se
lab head
Amir Ata Saei
contact affiliationDepartment of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact emailamirata.saei.dibavar@ki.se
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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