PXD014445

PXD014445 is an original dataset announced via ProteomeXchange.

Title	System-wide identification of enzyme substrates by thermal analysis (SIESTA)
Description	Despite the immense importance of enzyme-substrate reactions, there is a lack of generic and unbiased tools for identifying the molecular components participating in these reactions on a cellular level. Here we developed a universal method called System-wide Identification of Enzyme Substrates by Thermal Analysis (SIESTA). The approach assumes that enzymatic post-translational modification of substrate proteins changes their thermal stability, and applies the concept of specificity to reveal potential substrates. We have already shown the applicability of SIESTA in substrate discovery for TXNRD1 and PARP10 enzymes. SIESTA successfully identified several known and novel substrate candidates for protein kinase B (AKT1). A number of putative substrates were confirmed by functional assays. Wider application of SIESTA can enhance our understanding of the role of enzymes in homeostasis and disease, open new opportunities in investigating the effect of PTMs on protein stability, and facilitate drug discovery.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:17:43.377.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amir Ata Saei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 03:42:44	ID requested
1	2021-01-11 00:01:53	announced
2	2021-04-06 02:11:12	announced	2021-04-06: Updated publication reference for PubMed record(s): 33637753.
⏵ 3	2024-10-22 05:17:44	announced	2024-10-22: Updated project metadata.

Saei AA, Beusch CM, Sabatier P, Wells JA, Gharibi H, Meng Z, Chernobrovkin A, Rodin S, N, ä, reoja K, Thorsell AG, Karlberg T, Cheng Q, Lundstr, ö, m SL, Gaetani M, V, é, gv, á, ri Á, Arn, é, r ESJ, Sch, ü, ler H, Zubarev RA, System-wide identification and prioritization of enzyme substrates by thermal analysis. Nat Commun, 12(1):1296(2021) [pubmed]

10.1038/s41467-021-21540-6;

submitter keyword: proteomics, post-translational modification, target, thermal stability, LC/MS,AKT1, TrxR1, PARP10, ARTD10, RAC-alpha serine threonine protein kinase, TXNRD1

Roman Zubarev
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, STOCKHOLM, Sweden
contact email	roman.zubarev@ki.se
lab head
Amir Ata Saei
contact affiliation	Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
contact email	amirata.saei.dibavar@ki.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD014445

PRIDE project URI

• PRIDE
  1. PXD014445
     1. Label: PRIDE project
     2. Name: System-wide identification of enzyme substrates by thermal analysis (SIESTA)