PXD014435

PXD014435 is an original dataset announced via ProteomeXchange.

Title	OK-SnRK2 cascade mediates osmotic stress signaling in plant
Description	Osmotic stress associated with drought, salinity and cold is a major factor limits plant productivity. A few signaling factors have been identified in the sensing or early signaling of osmotic stress. SNF1-related protein kinase 2 (SnRK2) are quickly activated by osmotic stress and the snrk2 decuple are sensitive to hyperosmolality and defective in osmotic stress induced cellular responses. Mechanically activated ion channels Hyperosmolality-induced Ca2+ increases (OSCA) may involve in the sorbitol-evoked Ca2+ increase. However, how plant senses osmotic stress and quickly activates SnRK2s is still unclear. Here we report osmotic stress active protein kinases (OKs), a subfamily of Raf protein kinases, involved in early signaling of osmotic stress in plant. OKs quickly and specifically activated by multiple hyperosmolality, but not by phytohormone abscisic acid or cold stress. The high order mutants of oks were hypertensive to hyperosmolality. In addition, OKs physically interact with and phosphorylate SnRK2s in vivo and in vitro, and this phosphorylation is required for activation of SnRK2s upon osmotic stress. Our results indicated the OKs-SnRK2s kinase cascade in plant osmotic stress responses, which provide a potential molecular machinery mediates osmotic stress signaling in plant.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:27.276.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuan-Chih Hsu
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 01:39:14	ID requested
1	2020-01-17 00:17:13	announced
2	2020-02-17 03:20:28	announced	2020-02-17: Updated publication reference for PubMed record(s): 32001690.
⏵ 3	2024-10-22 04:05:30	announced	2024-10-22: Updated project metadata.

Lin Z, Li Y, Zhang Z, Liu X, Hsu CC, Du Y, Sang T, Zhu C, Wang Y, Satheesh V, Pratibha P, Zhao Y, Song CP, Tao WA, Zhu JK, Wang P, A RAF-SnRK2 kinase cascade mediates early osmotic stress signaling in higher plants. Nat Commun, 11(1):613(2020) [pubmed]

10.1038/s41467-020-14477-9;

submitter keyword: SnRK2, osmotic stress active protein kinases,Osmotic stress

Pengcheng Wang
contact affiliation	Shanghai Center for Plant Stress Biology, CAS Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences
contact email	pcwang@sibs.ac.cn
lab head
Chuan-Chih Hsu
contact affiliation	Academia Sinica
contact email	without1102@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014435
     1. Label: PRIDE project
     2. Name: OK-SnRK2 cascade mediates osmotic stress signaling in plant