PXD014433

PXD014433 is an original dataset announced via ProteomeXchange.

Title	RNA helicase DDX21 mediates nucleotide stress responses
Description	The stress of nucleotide pool reduction regulates transcription in neural crest and melanoma cells. To better understand the molecular response caused by nucleotide stress, we designed a chemical suppressor screen for leflunomide, an inhibitor of dihydroorate dehydrogenase. We found that alterations in the progesterone receptor (Pgr) activity suppressed the neural crest effects of leflunomide. To clarify the mechanism of action, we found that the RNA helicase protein, Ddx21, binds to Pgr, and loss of function of Ddx21 conferred resistance to nucleotide stress in zebrafish embryos. At the molecular level, nucleotide stress reduces DDX21 chromatin occupancy and thus, target gene expression. Together our results show that DDX21 is a transcriptional sensor and mediator of the nucleotide stress response.
HostingRepository	PRIDE
AnnounceDate	2020-02-06
AnnouncementXML	Submission_2020-09-28_00:07:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Marian Kalocsay
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2019-07-01 01:17:02	ID requested
1	2020-02-06 02:30:23	announced
⏵ 2	2020-09-28 00:07:33	announced	2020-09-28: Updated publication reference for PubMed record(s): 32231306.

Santoriello C, Sporrij A, Yang S, Flynn RA, Henriques T, Dorjsuren B, Custo Greig E, McCall W, Stanhope ME, Fazio M, Superdock M, Lichtig A, Adatto I, Abraham BJ, Kalocsay M, Jurynec M, Zhou Y, Adelman K, Calo E, Zon LI, RNA helicase DDX21 mediates nucleotide stress responses in neural crest and melanoma cells. Nat Cell Biol, 22(4):372-379(2020) [pubmed]

ProteomeXchange project tag: Cancer (B/D-HPP), Human Proteome Project

submitter keyword: DDX21, RNA, A375

Leonard Zon
contact affiliation	Stem Cell Program and Division of Hematology/Oncology Boston Children’s Hospital and Dana Farber Cancer Institute•Howard Hughes Medical Institute•Harvard Medical School•Harvard Stem Cell Institute•Stem Cell and Regenerative Biology Department Harvard University
contact email	zon@enders.tch.harvard.edu
lab head
Marian Kalocsay
contact affiliation	Harvard Medical School
contact email	marian_kalocsay@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD014433

PRIDE project URI

• PRIDE
  1. PXD014433
     1. Label: PRIDE project
     2. Name: RNA helicase DDX21 mediates nucleotide stress responses