PXD014426

PXD014426 is an original dataset announced via ProteomeXchange.

Title	ZapE/Afg1 is Oxa1-dependent regulator of respiratory complexes in trypanosomes
Description	Abstract ZapE/Afg1 is a component of the inner cell membrane of some eubacteria as well as in the inner mitochondrial membrane of eukaryotes. This protein is involved in FtsZ-dependent division of bacteria and facilitates the degradation of mitochondrion-encoded subunits of respiratory complexes. Furthermore, depletion of ZapE increases resistence to apoptosis, decreases oxidative stress tolerance, and impacts mitochondrial protein homeostasis. It is unclear whether this protein is a multifunctional protein or some observed phenotypes are a secondary consequence of respiratory chain disruption. Here, we have analyzed the functions of ZapE in Trypanosoma brucei, a parasitic protist and also an important model organism. Using a newly developed BioID2 proximity-dependent biotinylation approach, we have identified the inner mitochondrial membrane insertase Oxa1 as a direct interacting partner of ZapE, which is present in two paralogs. RNAi-mediated depletion of both ZapE paralogs affected the function of respiratory complexes I and IV. We propose that it is primarily the interaction of ZapE with Oxa1, which is required for a proper insertion of many inner mitochondrial membrane proteins, that is behind the multifaceted phenotype caused by the ablation of ZapE.
HostingRepository	PRIDE
AnnounceDate	2020-06-10
AnnouncementXML	Submission_2020-06-10_04:36:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Trypanosoma brucei brucei TREU927; NCBI TaxID: 185431;
ModificationList	biotinylated residue
Instrument	LTQ FT

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-28 07:46:50	ID requested
⏵ 1	2020-06-10 04:36:33	announced

Dataset with its publication pending

submitter keyword: Trypanosoma, BioID,

Jan Pyriha
contact affiliation	Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice (Budweis), Czech Republic
contact email	jan.pyrih@gmail.com
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014426
     1. Label: PRIDE project
     2. Name: ZapE/Afg1 is Oxa1-dependent regulator of respiratory complexes in trypanosomes