PXD014422

PXD014422 is an original dataset announced via ProteomeXchange.

Title	Proteome analysis of PC12 cells reveals alterations in translation regulation and actin signalling induced by clozapine
Description	Although antipsychotics are routinely used in the treatment of schizophrenia for last decades, their precise mechanism of action is still unclear. In this study we investigated changes in PC12 cells’ proteome under the influence of clozapine, risperidone and haloperidol to identify protein pathways regulated by the antipsychotics. Analysis of the protein profiles in two time points: after 12 and 24 h of incubation with drugs revealed significant alterations in 510 proteins. Further canonical pathway analysis determined signal transduction pathways and biological processes regulatednby drug treatment. Interestingly, all tested drugs have caused changes in PC12 proteome which correspond to inhibition of cytokines: tumor necrosis factor (TNF) and transforming growth factor beta 1 (TGF-β1), what can be linked to the immunological and viral hypothesis of schizophrenia. We found, that the 12-hour incubation with clozapine caused up-regulation of protein kinase A signalling and translation machinery. After 24 h of treatment with clozapine, the inhibition of the actin cytoskeleton signalling and Rho proteins signalling was revealed. Obtained results suggests that mammalian target of rapamycin complex 1 (mTORC1) and 2 (mTORC2) play a central role in the signal transduction of clozapine.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:26:27.289.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Urszula Jankowska
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-28 02:05:58	ID requested
1	2021-09-08 12:22:52	announced
⏵ 2	2024-10-22 05:26:28	announced	2024-10-22: Updated project metadata.

10.1007/s11064-021-03348-4;

Jankowska U, Skupien-Rabian B, Swiderska B, Prus G, Dziedzicka-Wasylewska M, Kedracka-Krok S, Proteome Analysis of PC12 Cells Reveals Alterations in Translation Regulation and Actin Signaling Induced by Clozapine. Neurochem Res, 46(8):2097-2111(2021) [pubmed]

submitter keyword: Proteome, Clozapine, Translation,Actin signalling, mTOR

Sylwia Kędracka-Krok
contact affiliation	Department of Physical Biochemistry, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Krakow, Poland
contact email	sylwia.kedracka-krok@uj.edu.pl
lab head
Urszula Jankowska
contact affiliation	Proteomics and Mass Spectrometry Core Facility, Malopolska Centre of Biotechnology, Jagiellonian University
contact email	urszula.jankowska@uj.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/09/PXD014422

PRIDE project URI

• PRIDE
  1. PXD014422
     1. Label: PRIDE project
     2. Name: Proteome analysis of PC12 cells reveals alterations in translation regulation and actin signalling induced by clozapine