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PXD014421

PXD014421 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA TetR-Family Protein (CAETHG_0459) Activates Transcription From a New Promoter Motif Associated With Essential Genes for Autotrophic Growth in Acetogens
DescriptionAcetogens can fix carbon (CO or CO2) into acetyl-CoA via the Wood-Ljungdahl pathway (WLP) that also makes them attractive cell factories for the production of fuels and chemicals from waste feedstocks. Although most biochemical details of the WLP are well understood and systems-level characterisation of acetogen metabolism has recently improved, key transcriptional features such as promoter motifs and transcriptional regulators are still unknown in acetogens. Here, we use differential RNA-sequencing to identify a previously undescribed promoter motif associated with essential genes for autotrophic growth of the model-acetogen Clostridium autoethanogenum. RNA polymerase was shown to bind to the new promoter motif using a DNA-binding protein assay and proteomics enabled the discovery of four candidates to potentially function directly in control of transcription of the WLP and other key genes of C1 fixation metabolism. Next, in vivo experiments showed that a TetR-family transcriptional regulator (CAETHG_0459) and the housekeeping sigma factor (??A) activate expression of a reporter protein (GFP) in-frame with the new promoter motif from a fusion vector in E. coli. Lastly, a protein-protein interaction assay with the RNA polymerase (RNAP) shows that CAETHG_0459 directly binds to the RNAP. Together, the data presented here advance the fundamental understanding of transcriptional regulation of C1 fixation in acetogens and provide a strategy for improving the performance of gas-fermenting bacteria by genetic engineering.
HostingRepositoryPRIDE
AnnounceDate2019-11-27
AnnouncementXMLSubmission_2020-01-14_07:01:47.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKaspar Valgepea
SpeciesList scientific name: Clostridium autoethanogenum; NCBI TaxID: 84023;
ModificationListiodoacetamide derivatized residue
InstrumentTripleTOF 5600; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-28 02:05:58ID requested
12019-11-20 06:45:46announced
22019-11-27 03:03:00announced2019-11-27: Updated project metadata.
32020-01-14 07:03:14announced2019-11-27: Updated project metadata.
2020-01-14: Updated publication reference for PubMed record(s): 31803150.
Publication List
de Souza Pinto Lemgruber R, Valgepea K, Gonzalez Garcia RA, de Bakker C, Palfreyman RW, Tappel R, Köpke M, Simpson SD, Nielsen LK, Marcellin E, A TetR-Family Protein (CAETHG_0459) Activates Transcription From a New Promoter Motif Associated With Essential Genes for Autotrophic Growth in Acetogens. Front Microbiol, 10():2549(2019) [pubmed]
Keyword List
submitter keyword: biological
data-dependent acquisition
gas fermentation
acetogen
Contact List
Esteban Marcellin
contact affiliationAustralian Institute for Bioengineering and Nanotechnolog (AIBN), The University of Queensland
contact emaile.marcellin@uq.edu.au
lab head
Kaspar Valgepea
contact affiliationAustralian Institute for Bioengineering and Nanotechnology,The University of Queensland
contact emailk.valgepea@uq.edu.au
dataset submitter
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