PXD014418

PXD014418 is an original dataset announced via ProteomeXchange.

Title	Phospho-proteomic Analyses Provide Insight into Underlying Molecular Mechanisms of NETosis
Description	NETosis, a novel cell death leads to neutrophil extracellular trap (NET) formation, is involved in both infectious and noninfectious disease. However, mechanisms underlying NETosis remain unclear. To explore the underlying molecular mechanisms and common factors associated with both NOX-dependent and NOX-independent NETosis, we conducted global proteomics and phospho-proteomics analyses of phorbol 12-myristate 13-acetate (PMA)-, ionomycin-, and monosodium urate (MSU) - induced early stage NETosis. Global proteomic analyses identified 64, 97, and 141 proteins differentially regulated in the PMA, ionomycin, and MSU comparisons with the control, respectively. Next, phospho-proteomic analyses identified: 931, 565 and 201 phosphorylation sites differentially regulated in the PMA, ionomycin, and MSU comparisons with the control, respectively. Furthermore, overlap analysis of the three comparisons identified 9 proteins and 49 phosphorylation sites derived from 41 phosphoproteins. Among the 41 differentially regulated phosphoproteins, 23 were associated with the nucleus, 5 with chromatin binding and 13 with poly(A) RNA binding according to GO annotation. Of which, DEK, Methyl-CpG-binding protein 2 (MECP2) and structure-specific recognition protein 1 (SSRP1) involved in both chromatin and poly(A) RNA binding. In conclusion, our study provides insight into molecular mechanisms of NETosis and this dataset will be benefit for further elucidation.
HostingRepository	iProX
AnnounceDate	2019-06-27
AnnouncementXML	Submission_2023-08-27_21:08:00.439.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaomei Li
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-27 19:49:45	ID requested
⏵ 1	2023-08-27 21:08:01	announced

Zhu X, Chen J, Phosphoproteomic Analyses Provide Insight into Molecular Mechanisms Underlying NETosis. Proteomics, 19(19):e1900126(2019) [pubmed]

submitter keyword: Neutraphil extracellular traps, NETosis, phospho-proteomics, proteomics

Jinjun Chen
contact affiliation	Department of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University
contact email	chjj@smu.edu.cn
lab head
Xiaomei Li
contact affiliation	Jingjie PTM BIO (Hangzhou, China) Co., Ltd
contact email	xiaomei_li@ptm-biolab.com
dataset submitter

iProX dataset URI