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PXD014418

PXD014418 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhospho-proteomic Analyses Provide Insight into Underlying Molecular Mechanisms of NETosis
DescriptionNETosis, a novel cell death leads to neutrophil extracellular trap (NET) formation, is involved in both infectious and noninfectious disease. However, mechanisms underlying NETosis remain unclear. To explore the underlying molecular mechanisms and common factors associated with both NOX-dependent and NOX-independent NETosis, we conducted global proteomics and phospho-proteomics analyses of phorbol 12-myristate 13-acetate (PMA)-, ionomycin-, and monosodium urate (MSU) - induced early stage NETosis. Global proteomic analyses identified 64, 97, and 141 proteins differentially regulated in the PMA, ionomycin, and MSU comparisons with the control, respectively. Next, phospho-proteomic analyses identified: 931, 565 and 201 phosphorylation sites differentially regulated in the PMA, ionomycin, and MSU comparisons with the control, respectively. Furthermore, overlap analysis of the three comparisons identified 9 proteins and 49 phosphorylation sites derived from 41 phosphoproteins. Among the 41 differentially regulated phosphoproteins, 23 were associated with the nucleus, 5 with chromatin binding and 13 with poly(A) RNA binding according to GO annotation. Of which, DEK, Methyl-CpG-binding protein 2 (MECP2) and structure-specific recognition protein 1 (SSRP1) involved in both chromatin and poly(A) RNA binding. In conclusion, our study provides insight into molecular mechanisms of NETosis and this dataset will be benefit for further elucidation.
HostingRepositoryiProX
AnnounceDate2019-06-27
AnnouncementXMLSubmission_2023-08-27_21:08:00.439.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXiaomei Li
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-27 19:49:45ID requested
12023-08-27 21:08:01announced
Publication List
Zhu X, Chen J, Phosphoproteomic Analyses Provide Insight into Molecular Mechanisms Underlying NETosis. Proteomics, 19(19):e1900126(2019) [pubmed]
Keyword List
submitter keyword: Neutraphil extracellular traps, NETosis, phospho-proteomics, proteomics
Contact List
Jinjun Chen
contact affiliationDepartment of Infectious Diseases and Hepatology Unit, Nanfang Hospital, Southern Medical University
contact emailchjj@smu.edu.cn
lab head
Xiaomei Li
contact affiliationJingjie PTM BIO (Hangzhou, China) Co., Ltd
contact emailxiaomei_li@ptm-biolab.com
dataset submitter
Full Dataset Link List
iProX dataset URI