PXD014416 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Spatiotemporal proteomics of hormone treated mice (urine across time, prostate tissue) |
Description | Benign prostatic hyperplasia and related lower urinary tract symptoms remain common, costly, and impactful issues for aging males. Etiology and pathogenesis are multifactorial and include steroid hormone changes and inflammation. Noninvasive markers could one day inform personalized medicine, but interindividual variation and lack of healthy age-matched controls hamper research. Experimental models are appealing for insight into disease mechanisms. Here, we present a spatiotemporal proteomics study in a mouse model of hormone-induced urinary dysfunction. Urine samples were collected noninvasively across time: before, during, and after disease onset. Microcomputed tomography analysis implicated the prostate as a spatially relevant contributor to bladder outlet obstruction. Prostates were collected after disease onset and compared with control mice. Notable changes in urine include proteins representing oxidative stress defense and acute phase inflammatory response processes. In the prostate, hormone treatment led to perturbations related to oxidative stress response and H2O2 metabolism. Several protein changes coincided in both urine and prostate tissue, including Ctsb, Qsox1, and Gpx3. This study supports the concept of noninvasive urinary biomarkers for prostate disease diagnostics. Oxidative stress and acute phase inflammatory processes were identified as key consequences of hormone-induced bladder outlet obstruction. Future research into antioxidants and anti-inflammatories in prostate disease appears promising. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:26:07.852.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Samuel Thomas |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-27 09:12:54 | ID requested | |
1 | 2021-09-08 10:30:54 | announced | |
⏵ 2 | 2024-10-22 05:26:08 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1021/acs.jproteome.9b00451; |
Thomas S, Hao L, DeLaney K, McLean D, Steinke L, Marker PC, Vezina CM, Li L, Ricke WA, Spatiotemporal Proteomics Reveals the Molecular Consequences of Hormone Treatment in a Mouse Model of Lower Urinary Tract Dysfunction. J Proteome Res, 19(4):1375-1382(2020) [pubmed] |
Keyword List
submitter keyword: Mus musculus, urine, proteomics, hormone, prostate |
Contact List
Lingjun Li |
contact affiliation | University of Wisconsin - Madison |
contact email | lingjun.li@wisc.edu |
lab head | |
Samuel Thomas |
contact affiliation | University of Wisconsin - Madison |
contact email | sdthomas2@wisc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014416
- Label: PRIDE project
- Name: Spatiotemporal proteomics of hormone treated mice (urine across time, prostate tissue)