PXD014404

PXD014404 is an original dataset announced via ProteomeXchange.

Title	Screening of Potential Biomarkers with Diagnostic Value in Gastric Cancer Using Label-free Global Proteome Analysis
Description	Gastric cancer (GC) is one of the most common malignant tumors worldwide. Many patients diagnosed with GC are already at a late stage. Despite the decrease in mortality rates recently, the prognosis of this cancer is poor. Therefore, it is necessary to explore novel biomarkers with early diagnostic value with GC. In this study, we present a large-scale proteomic analysis of 30 GC tissues and 30 matched normal tissues using label-free global proteome profiling. The results allowed identifying 537 differentially expressed proteins, including 280 upregulated and 257 downregulated proteins. The ingenuity pathway analysis (IPA) results indicated that the sirtuin signaling pathway was the most activated whereas the oxidative phosphorylation was the most inhibited pathway. Moreover, the most activated molecular function was cell movement, including tissue invasion by tumor cell lines. Based on IPA results, 15 hub proteins were screened. Using the receiver operating characteristic curve, most of these hub proteins had a high diagnostic power in distinguishing tumors and normal controls. A four-protein (ATP5B-ATP5O-NDUFB4-NDUFB8) diagnostic signature was built using a random forest model. The area under curve (AUC) of this model was 0.864 in the validation set, suggesting a high diagnostic power. In the testing set of plasma enzyme-linked immune sorbent assay, the results achieved an AUC of 0.778 and accuracy of 71.8% to distinguish GC tissues from healthy controls. In conclusion, this study identified potential biomarkers and help to understand the pathogenesis of GC and provide novel and specific therapeutic targets for this cancer.
HostingRepository	iProX
AnnounceDate	2019-06-26
AnnouncementXML	Submission_2022-11-27_22:33:06.111.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhenning Wang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-26 19:41:37	ID requested
⏵ 1	2022-11-27 22:33:06	announced

Song Y, Wang J, Sun J, Chen X, Shi J, Wu Z, Yu D, Zhang F, Wang Z, Screening of Potential Biomarkers for Gastric Cancer with Diagnostic Value Using Label-free Global Proteome Analysis. Genomics Proteomics Bioinformatics, 18(6):679-695(2020) [pubmed]

submitter keyword: Gastric cancer,Proteomics, Label-free,Bioinformatics,Diagnosis,Biomarkers,Prediction

Zhenning Wang
contact affiliation	The First Hospital of China Medical University
contact email	josieon826@sina.cn
lab head
Zhenning Wang
contact affiliation	The First Hospital of China Medical University
contact email	josieon826@sina.cn
dataset submitter

iProX dataset URI