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PXD014400

PXD014400 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInsoluble fraction_Heat stress_S.pombe LC-MSMS
DescriptionWe have carried out proteomic studies to identify the thermo-unstable proteome fraction in fission yeast. We have performed biochemical separation of pellet fractions by centrifugation of whole cell extracts from wild-type cultures grown at different temperatures, and performed untagged liquid chromatography coupled to tandem MS (LC-MS/MS) of biological triplicates. We demonstrate that formation of protein aggregates during heat shock is a chaperone-mediated adaptation strategy which occurs with thermo-unstable endogenous proteins as well.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:05:47.803.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSusanna Boronat
SpeciesList scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap XL
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-26 05:42:35ID requested
12020-02-23 23:39:11announced
22024-10-22 04:05:55announced2024-10-22: Updated project metadata.
Publication List
10.1016/j.celrep.2020.01.077;
Cabrera M, Boronat S, Marte L, Vega M, P, é, rez P, Ayt, é J, Hidalgo E, Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress. Cell Rep, 30(7):2430-2443.e4(2020) [pubmed]
Keyword List
submitter keyword: LC-MSMS, Aggregates,S. pombe, Heat stress, Insoluble protein
Contact List
Elena Hidalgo
contact affiliationOxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, 08003 Barcelona, Spain
contact emailelena.hidalgo@upf.edu
lab head
Susanna Boronat
contact affiliationUniversitat Pompeu Fabra
contact emailsusanna.boronat@upf.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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