PXD014400

PXD014400 is an original dataset announced via ProteomeXchange.

Title	Insoluble fraction_Heat stress_S.pombe LC-MSMS
Description	We have carried out proteomic studies to identify the thermo-unstable proteome fraction in fission yeast. We have performed biochemical separation of pellet fractions by centrifugation of whole cell extracts from wild-type cultures grown at different temperatures, and performed untagged liquid chromatography coupled to tandem MS (LC-MS/MS) of biological triplicates. We demonstrate that formation of protein aggregates during heat shock is a chaperone-mediated adaptation strategy which occurs with thermo-unstable endogenous proteins as well.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:47.803.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Susanna Boronat
SpeciesList	scientific name: Schizosaccharomyces pombe 927; NCBI TaxID: 1264690;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-26 05:42:35	ID requested
1	2020-02-23 23:39:11	announced
⏵ 2	2024-10-22 04:05:55	announced	2024-10-22: Updated project metadata.

10.1016/j.celrep.2020.01.077;

Cabrera M, Boronat S, Marte L, Vega M, P, é, rez P, Ayt, é J, Hidalgo E, Chaperone-Facilitated Aggregation of Thermo-Sensitive Proteins Shields Them from Degradation during Heat Stress. Cell Rep, 30(7):2430-2443.e4(2020) [pubmed]

submitter keyword: LC-MSMS, Aggregates,S. pombe, Heat stress, Insoluble protein

Elena Hidalgo
contact affiliation	Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/ Dr. Aiguader 88, 08003 Barcelona, Spain
contact email	elena.hidalgo@upf.edu
lab head
Susanna Boronat
contact affiliation	Universitat Pompeu Fabra
contact email	susanna.boronat@upf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/02/PXD014400

PRIDE project URI

• PRIDE
  1. PXD014400
     1. Label: PRIDE project
     2. Name: Insoluble fraction_Heat stress_S.pombe LC-MSMS