PXD014383

PXD014383 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of isolated mouse distal convoluted tubules following high potassium or low sodium diet
Description	The “aldosterone paradox” is a poorly understood physiological mechanism in which increases in the hormone aldosterone either serve to stabilize blood pressure by increasing sodium-chloride (NaCl) reabsorption or to excrete potassium (K+). Under low NaCl intake, abundance and activity of the thiazide-sensitive NaCl cotransporter NCC, expressed in the distal convoluted tubule (DCT), is high, whereas it is low during high K+ intake - leading to increased delivery of NaCl to downstream segments and increased electrogenic K+ secretion. Despite a critical role in blood pressure and K+ homeostasis, little is known about the molecular alterations in the DCT during the aldosterone paradox, and how they control NCC levels. The objective of this study was to define the proteome of the DCT and how it is modified by increased circulating aldosterone levels subsequent to long-term low dietary NaCl or high K+ intake.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:20:34.767.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Qi Wu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-25 09:01:01	ID requested
1	2021-03-30 12:07:18	announced
⏵ 2	2024-10-22 05:20:35	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: distal convoluted tubule, potassium,Mouse, sodium, kidney, aldosterone

Robert A. Fenton
contact affiliation	InterPrET Center, Department of Biomedicine, Aarhus University, Aarhus, Denmark
contact email	robert.a.fenton@biomed.au.dk
lab head
Qi Wu
contact affiliation	Department of Biomedicine, Aarhus University
contact email	qi.wu@biomed.au.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014383
     1. Label: PRIDE project
     2. Name: Proteomic analysis of isolated mouse distal convoluted tubules following high potassium or low sodium diet