PXD014380

PXD014380 is an original dataset announced via ProteomeXchange.

Title	Novel mechanisms for phosphate acquisition in abundant rhizosphere-dwelling Bacteroidetes
Description	Global food production is reliant on the application of finite phosphorus (P) fertilisers. Numerous negative consequences associated with intensive P fertilisation have resulted in a high demand to find alternative sustainable methods that will enhance crop P uptake. Bacteroidetes, primarily from the genus Flavobacterium, have recently been shown to be abundant members of the plant microbiome, but their general ecological role and potential to mobilise P in the rhizosphere remains very poorly characterised. Here, we sought to determine the P mobilisation potential of Flavobacterium strains isolated from the rhizosphere of oilseed rape (Brassica napus L.). In contrast to other abundant rhizosphere bacteria, such as Pseudomonas, all Flavobacterium strains exhibited constitutive phosphatase activity independent of external phosphate (Pi) concentrations. Interestingly, a combination of exoproteomic analysis and molecular microbiology techniques revealed that Flavobacterium have a complex and largely unique repertoire of proteins to mobilise and acquire Pi. This includes the expression of novel, as yet unidentified, phosphatases, and numerous proteins of unknown function. We also discovered that Flavobacterium expresses certain SusCD-like transporters, whose role is typically associated with specialised carbon acquisition, in response to Pi-starvation. Furthermore, the genes encoding these unusual Pi-responsive proteins were enriched in plant-associated Flavobacterium strains suggesting that this machinery represents niche-adaptive strategies for overcoming P scarcity in this genus. We propose that abundant rhizosphere-dwelling Flavobacterium spp. have evolved unique mechanisms for coping with Pi-stress which may provide novel solutions for future sustainable agricultural practices.
HostingRepository	PRIDE
AnnounceDate	2021-09-08
AnnouncementXML	Submission_2021-09-08_11:05:13.292.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014380
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Andrew Bottrill
SpeciesList	scientific name: Flavobacterium sp. 316; NCBI TaxID: 1603293;
ModificationList	Oxidation; Acetyl; Carbamidomethyl
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-25 06:25:46	ID requested
⏵ 1	2021-09-08 11:05:14	announced

Lidbury IDEA, Borsetto C, Murphy ARJ, Bottrill A, Jones AME, Bending GD, Hammond JP, Chen Y, Wellington EMH, Scanlan DJ, Niche-adaptation in plant-associated Bacteroidetes favours specialisation in organic phosphorus mineralisation. ISME J, 15(4):1040-1055(2021) [pubmed]

submitter keyword: Exoproteomics, Bacteroidetes, Rhizosphere, Oilseed Rape

Professor Elizabeth M. H. Wellington
contact affiliation	School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, UK
contact email	E.M.H.Wellington@warwick.ac.uk
lab head
Andrew Bottrill
contact affiliation	University of Warwick
contact email	a.r.bottrill@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014380
     1. Label: PRIDE project
     2. Name: Novel mechanisms for phosphate acquisition in abundant rhizosphere-dwelling Bacteroidetes