PXD014358

PXD014358 is an original dataset announced via ProteomeXchange.

Title	Zygote/2-celled proembryo specific protein TaMAB2 is involved in proteosomal degradation of translation initiation factors via a cytoskeleton related mechanism
Description	A family of MATH-BTB proteins is known to act as substrate-specific adaptors of CUL3-based E3 ligases in the ubiquitin proteasome pathway. The BTB domain binds a CUL3 scaffold protein and the less conserved MATH domain targets a highly diverse collection of substrate proteins to promote their ubiquitination and subsequent degradation. Among plants, a significant expansion of the MATH-BTB family occurred in grasses. Here, we functionally describe TaMAB2, a wheat MATH-BTB protein expressed exclusively in the zygote and two-celled proembryo. The overexpression of TaMAB2 in Arabidopsis plants provoked microtubular bundling and re-orientation together with retarded growth. In model BY-2 cells, TaMAB2 showed a microtubule and a ubiquitin-related localization which together with direct interaction with CUL3 pointed out its function in targeting specific substrates for ubiquitin-dependent degradation. Results of protein interaction analyses indicated eukaryotic translation initiation factors 3 and 4 as the most likely substrates of TaMAB2, while the interaction with cytoskeletal element, particularly actin 11 hints at the possible TaMAB2 mediated intreplay between cytoskeleton and translation initiation machinery during the first division of the zygote.
HostingRepository	PRIDE
AnnounceDate	2019-10-24
AnnouncementXML	Submission_2019-12-11_07:03:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Genadij Razdorov
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-24 06:19:11	ID requested
1	2019-10-24 05:36:30	announced
⏵ 2	2019-12-11 07:03:57	announced	2019-12-11: Updated publication reference for DOI(s): 10.3389/fpls.2019.01469.

10.3389/fpls.2019.01469;

submitter keyword: MATH-BTB, translation initiation, eIF3, eIF4, cytoskeleton, actin11, proteasomal degradation, ubiquitin, Triticum aestivum, LC-MS/MS

Dunja Leljak
contact affiliation	Division of Molecular Biology, Department of Biology, Faculty of Science, University of Zagreb, Croatia
contact email	dunja@zg.biol.pmf.hr
lab head
Genadij Razdorov
contact affiliation	Genos ltd
contact email	genadijrazdorov@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/10/PXD014358

PRIDE project URI

• PRIDE
  1. PXD014358
     1. Label: PRIDE project
     2. Name: Zygote/2-celled proembryo specific protein TaMAB2 is involved in proteosomal degradation of translation initiation factors via a cytoskeleton related mechanism