PXD014349

PXD014349 is an original dataset announced via ProteomeXchange.

Title	Pyrin oligomerization nucleates antiviral IFI16 sensing of herpesvirus DNA
Description	The formation of multimerized protein assemblies has emerged as a core component of immune signal amplification, yet the biochemical basis of this phenomenon remains unclear for many mammalian proteins within host defense pathways. The interferon-inducible protein 16 (IFI16) is a viral DNA sensor that oligomerizes upon binding to nuclear viral DNA and induces downstream antiviral responses. Here, we first generated oligomerization-incompetent IFI16 mutants that exhibit severely reduced ability to induce antiviral cytokine expression, suppress herpes simplex virus 1 (HSV-1) protein levels, and restrict viral progeny production. Using immunoaffinity purification and targeted mass spectrometry, we establish that oligomerization promotes IFI16 interactions with several proteins involved in transcriptional regulation, including PAF1C, UBTF, and ND10
HostingRepository	PRIDE
AnnounceDate	2019-07-10
AnnouncementXML	Submission_2019-07-29_07:28:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tim Howard
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-24 02:37:11	ID requested
1	2019-07-10 01:39:48	announced
⏵ 2	2019-07-29 07:28:59	announced	Updated publication reference for PubMed record(s): 31337724.

Lum KK, Howard TR, Pan C, Cristea IM, Charge-Mediated Pyrin Oligomerization Nucleates Antiviral IFI16 Sensing of Herpesvirus DNA. mBio, 10(4):(2019) [pubmed]

submitter keyword: IP-MS, IFI16, HSV-1, oligomerization, DNA sensing

Ileana M Cristea
contact affiliation	Department of Molecular Biology, Princeton University
contact email	icristea@princeton.edu
lab head
Tim Howard
contact affiliation	Princeton University
contact email	th12@princeton.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014349
     1. Label: PRIDE project
     2. Name: Pyrin oligomerization nucleates antiviral IFI16 sensing of herpesvirus DNA