PXD014348

PXD014348 is an original dataset announced via ProteomeXchange.

Title	General structural features that regulate integrin affinity revealed by atypical αVβ8
Description	Integrins αVβ8 and αVβ6 are master activators of proTGF-βs. Whereas most integrins including αVβ6 are activated by tensile force applied by the cytoskeleton, αVβ8 links differently to the cytoskeleton and lacks the open headpiece conformation that represents the high affinity state of other integrins. Here, we shed light on the atypical activation mechanism of integrin αVβ8 using crystal structures in the absence and presence of proTGF-β1 peptide, comparisons of the hydrogen deuterium exchange dynamics of αVβ8 and αVβ6, and affinity measurements on mutants in which structurally atypical residues of αVβ8 and typical residues of αVβ6 were reciprocally exchanged.
HostingRepository	PRIDE
AnnounceDate	2019-12-03
AnnouncementXML	Submission_2020-01-13_05:23:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	John R. Engen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-24 02:35:02	ID requested
1	2019-12-03 12:13:40	announced
⏵ 2	2020-01-13 05:23:20	announced	2020-01-13: Updated publication reference for PubMed record(s): 31792290.

Wang J, Su Y, Iacob RE, Engen JR, Springer TA, 8. Nat Commun, 10(1):5481(2019) [pubmed]

submitter keyword: Integrin, proTGF-β, Crystal structure, Affinity, Conformational change, hydrogen deuterium exchange

John R. Engen
contact affiliation	Chemistry & Chemical Biology, Northeastern University
contact email	j.engen@northeastern.edu
lab head
John R. Engen
contact affiliation	Northeastern University
contact email	j.engen@northeastern.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/12/PXD014348

PRIDE project URI

• PRIDE
  1. PXD014348
     1. Label: PRIDE project
     2. Name: General structural features that regulate integrin affinity revealed by atypical αVβ8