PXD014343 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Qualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep |
Description | We developed a large animal model, with clinically relevant gravitational loading on the lymphatic vasculature, that enables longitudinal tracking of lymphatic function in vivo as well as end-point analysis of vessel function and mechanics. One of two parallel lymphatic vessels in the sheep hind limb was ligated with the contralateral limb acting as an internal control. To determine the changes in the protein expression profiles in the lymphatic muscle cells from the remodeled vessel compared to the control, we expanded isolated LMC in vitro and performed bottom-up proteomic analysis using a Q Exactive Plus mass spectrometer. Label-free quantitative (LFQ) methods directly used the raw spectral data from parallel MS runs to determine relative protein abundances. We used both “MS/MS (MS2) spectral counting” and “precursor MS1 area” methods for label free quantitation and further contrasting the differentially expressed proteomic profiles across the control and remodeled lymphatic muscle samples to determine the alterations in mitochondrial dysfunction and elevated oxidative stress within the lymphatic muscle. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-12 |
AnnouncementXML | Submission_2021-01-12_00:07:57.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD014343 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Cristina Clement |
SpeciesList | scientific name: Ovis aries; NCBI TaxID: 9940; |
ModificationList | Glu->pyro-Glu; Deamidated; Oxidation; Carbamidomethyl; Gln->pyro-Glu |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2019-06-21 04:47:10 | ID requested | |
⏵ 1 | 2021-01-12 00:07:58 | announced | |
Publication List
Nelson TS, Nepiyushchikh Z, Hooks JST, Razavi MS, Lewis T, Clement CC, Thoresen M, Cribb MT, Ross MK, Gleason RL, Santambrogio L, Peroni JF, Dixon JB, Lymphatic remodelling in response to lymphatic injury in the hind limbs of sheep. Nat Biomed Eng, 4(6):649-661(2020) [pubmed] |
Keyword List
submitter keyword: sheep lymphatic muscle cells |
Contact List
Brandon Dixon PhD |
contact affiliation | Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology 315 Ferst Dr. Atlanta, GA 30332 |
contact email | dixon@gatech.edu |
lab head | |
Cristina Clement |
contact affiliation | Weill Cornell Medicine |
contact email | ccc4002@med.cornell.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD014343
- Label: PRIDE project
- Name: Qualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep