PXD014343

PXD014343 is an original dataset announced via ProteomeXchange.

Title	Qualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep
Description	We developed a large animal model, with clinically relevant gravitational loading on the lymphatic vasculature, that enables longitudinal tracking of lymphatic function in vivo as well as end-point analysis of vessel function and mechanics. One of two parallel lymphatic vessels in the sheep hind limb was ligated with the contralateral limb acting as an internal control. To determine the changes in the protein expression profiles in the lymphatic muscle cells from the remodeled vessel compared to the control, we expanded isolated LMC in vitro and performed bottom-up proteomic analysis using a Q Exactive Plus mass spectrometer. Label-free quantitative (LFQ) methods directly used the raw spectral data from parallel MS runs to determine relative protein abundances. We used both “MS/MS (MS2) spectral counting” and “precursor MS1 area” methods for label free quantitation and further contrasting the differentially expressed proteomic profiles across the control and remodeled lymphatic muscle samples to determine the alterations in mitochondrial dysfunction and elevated oxidative stress within the lymphatic muscle.
HostingRepository	PRIDE
AnnounceDate	2021-01-12
AnnouncementXML	Submission_2021-01-12_00:07:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014343
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Cristina Clement
SpeciesList	scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationList	Glu->pyro-Glu; Deamidated; Oxidation; Carbamidomethyl; Gln->pyro-Glu
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-21 04:47:10	ID requested
⏵ 1	2021-01-12 00:07:58	announced

Nelson TS, Nepiyushchikh Z, Hooks JST, Razavi MS, Lewis T, Clement CC, Thoresen M, Cribb MT, Ross MK, Gleason RL, Santambrogio L, Peroni JF, Dixon JB, Lymphatic remodelling in response to lymphatic injury in the hind limbs of sheep. Nat Biomed Eng, 4(6):649-661(2020) [pubmed]

submitter keyword: sheep lymphatic muscle cells

Brandon Dixon PhD
contact affiliation	Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology 315 Ferst Dr. Atlanta, GA 30332
contact email	dixon@gatech.edu
lab head
Cristina Clement
contact affiliation	Weill Cornell Medicine
contact email	ccc4002@med.cornell.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014343
     1. Label: PRIDE project
     2. Name: Qualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep