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PXD014343

PXD014343 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQualitative and label-free quantitative analysis of protein changes in the lymphatic muscle cells isolated in a hind-limb lymphatic injury model in the sheep
DescriptionWe developed a large animal model, with clinically relevant gravitational loading on the lymphatic vasculature, that enables longitudinal tracking of lymphatic function in vivo as well as end-point analysis of vessel function and mechanics. One of two parallel lymphatic vessels in the sheep hind limb was ligated with the contralateral limb acting as an internal control. To determine the changes in the protein expression profiles in the lymphatic muscle cells from the remodeled vessel compared to the control, we expanded isolated LMC in vitro and performed bottom-up proteomic analysis using a Q Exactive Plus mass spectrometer. Label-free quantitative (LFQ) methods directly used the raw spectral data from parallel MS runs to determine relative protein abundances. We used both “MS/MS (MS2) spectral counting” and “precursor MS1 area” methods for label free quantitation and further contrasting the differentially expressed proteomic profiles across the control and remodeled lymphatic muscle samples to determine the alterations in mitochondrial dysfunction and elevated oxidative stress within the lymphatic muscle.
HostingRepositoryPRIDE
AnnounceDate2021-01-12
AnnouncementXMLSubmission_2021-01-12_00:07:57.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD014343
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterCristina Clement
SpeciesList scientific name: Ovis aries; NCBI TaxID: 9940;
ModificationListGlu->pyro-Glu; Deamidated; Oxidation; Carbamidomethyl; Gln->pyro-Glu
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-21 04:47:10ID requested
12021-01-12 00:07:58announced
Publication List
Nelson TS, Nepiyushchikh Z, Hooks JST, Razavi MS, Lewis T, Clement CC, Thoresen M, Cribb MT, Ross MK, Gleason RL, Santambrogio L, Peroni JF, Dixon JB, Lymphatic remodelling in response to lymphatic injury in the hind limbs of sheep. Nat Biomed Eng, 4(6):649-661(2020) [pubmed]
Keyword List
submitter keyword: sheep lymphatic muscle cells
Contact List
Brandon Dixon PhD
contact affiliationParker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology 315 Ferst Dr. Atlanta, GA 30332
contact emaildixon@gatech.edu
lab head
Cristina Clement
contact affiliationWeill Cornell Medicine
contact emailccc4002@med.cornell.edu
dataset submitter
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Dataset FTP location
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