PXD014338

PXD014338 is an original dataset announced via ProteomeXchange.

Title	A protein expression during development of S. coelicolor in liquid medium
Description	In this paper we analyzed the S.coelicolor proteome at four points in time as it grew in liquid medium s i using three biological and two technical replicates. The proteome was measured during exponential growth and its entry into the stationary phase at 20, 35, 50 and 65 hours. Differential expression analysis identified key metabolic pathways and their hierarchy of activation during population growth. 4 and 3 pathways were found significantly overrepresented only under 35 to 20 hours or 50 to 35 hours respectively. Six pathways were found to be overrepresented within all three temporal groups (20 to 35 hours, 35 to 50 hours and 50 to 65 hours), with one pathway being significantly overrepresented exclusively in the 50-65 h interval. Central metabolic system enzymes involved in DNA replication were enriched in the late stages while the proteins involved in the transcription and translation machinery were overrepresented in early stages and decreased over time. A strong correlation was found between the expression of about a third of the proteins and mRNA, while another third correlated negatively. The data presented here represents quantitative evidence for the growing populations’ observed behaviour.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:02:45.431.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Martin Hubalek
SpeciesList	scientific name: Streptomyces coelicolor; NCBI TaxID: 1902;
ModificationList	iodoacetamide derivatized residue; deamidated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-21 03:50:37	ID requested
1	2020-04-28 04:12:26	announced
⏵ 2	2024-10-22 05:02:50	announced	2024-10-22: Updated project metadata.

Posp, í, š, il J, Strunin D, Zikov, á A, Hub, á, lek M, Vohradsk, ý J, A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote (S. coelicolor). Proteomics, 20(14):e2000032(2020) [pubmed]

10.1002/pmic.202000032;

submitter keyword: SWATH MS

Matin Hubalek
contact affiliation	Institute of Organic Chemistry and Biochemistry Czech Academy of Sciences
contact email	hubalek@uoch.cas.cz
lab head
Martin Hubalek
contact affiliation	Institute of Organic Chemistry and Biochemistry, Czech Academy of Science
contact email	hubalek@uochb.cas.cz
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD014338
     1. Label: PRIDE project
     2. Name: A protein expression during development of S. coelicolor in liquid medium