PXD014329

PXD014329 is an original dataset announced via ProteomeXchange.

Title	Targeting of Tumor Cells by the Adenovirus E4orf4 protein via Par3 Polarity Protein-Regulated Nuclear Envelope Rupture
Description	The protein product of the Early region 4 open reading frame 4 (E4orf4) of human adenovirus 2 is reported to exhibit a tumor-selective cell killing activity. Here, we show that E4orf4’s tumoricidal activity correlated with changes in perinuclear actomyosin contractility that are controlled by an interaction with the Par3 polarity protein. Surprisingly, E4orf4 enhanced apical-basal polarity in non-transformed mammary epithelial cells. In contrast, in tumorigenic cells, it induced a high incidence of actin-dependent nuclear bleb rupture. This process, which was regulated by the linker of nucleoskeleton and cytoskeleton (LINC) complex, promoted nuclear efflux of E4orf4 and the breakdown of the nuclear compartment. Significantly, Par3 also regulated the incidence of transient nuclear envelope rupture due to the reduced nuclear rigidity in cells depleted of lamins, in absence of E4orf4. Thus, using E4orf4 as a probing tool, we uncovered a so far un-appreciated role for Par3 in controlling the actin-dependent forces acting on the nuclear envelope to remodel nuclear shape, which might be a defining feature of tumor cells that is harnessed by E4orf4.
HostingRepository	PRIDE
AnnounceDate	2020-03-19
AnnouncementXML	Submission_2020-06-12_01:44:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD014329
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kevin Jacquet
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; Deamidated; Oxidation; Carbamidomethyl
Instrument	TripleTOF 5600; QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2019-06-21 01:10:57	ID requested
1	2020-03-19 04:04:03	announced
⏵ 2	2020-06-12 01:44:11	announced	2020-06-12: Updated publication reference for PubMed record(s): 32328642.

Dziengelewski C, Rodrigue MA, Caillier A, Jacquet K, Boulanger MC, Bergeman J, Fuchs M, Lambert H, Laprise P, Richard DE, Bordeleau F, Huot M É, Lavoie JN, Adenoviral protein E4orf4 interacts with the polarity protein Par3 to induce nuclear rupture and tumor cell death. J Cell Biol, 219(4):(2020) [pubmed]

submitter keyword: E4orf4, polarity proteins, actin, live cell dynamics, nuclear bleb, lamin A/C

Josée, N., Lavoie
contact affiliation	Centre de Recherche sur le Cancer de l‘Université Laval,Oncology, Centre de Recherche du CHU de Québec-Université Laval, Canada, G1R 3S3
contact email	Josee.Lavoie@crchudequebec.ulaval.ca
lab head
Kevin Jacquet
contact affiliation	CRC quebec
contact email	kevin.jacquet.labobisson@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/03/PXD014329

PRIDE project URI

• PRIDE
  1. PXD014329
     1. Label: PRIDE project
     2. Name: Targeting of Tumor Cells by the Adenovirus E4orf4 protein via Par3 Polarity Protein-Regulated Nuclear Envelope Rupture