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PXD014318

PXD014318 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleUBB Pseudogene 4 encodes novel ubiquitin variants
DescriptionPost-translational modification by ubiquitin and ubiquitin-like modifiers proteins regulate cellular processes at almost every levels. Ubiquitin itself is encoded by four different genes, either as single copy of ubiquitin fused to ribosomal proteins, or by polyubiquitin precursors3. Early studies identified several additional genes potentially coding for ubiquitin, but they were considered as pseudogenes due to differences in amino acids, or lack of apparent transcription4-6. Through analysis of large-scale proteomics and RNA sequencing experiments, we found evidence for expression at the mRNA and protein levels of several ubiquitin pseudogenes. Our results show that UBBP4, a pseudogene of the UBB subfamily, produces functional ubiquitin proteins with different amino acids composition compared to the canonical sequence. These ubiquitins variants are covalently conjugated to proteins that are different from ubiquitin, and proteins modified by UBBP4 are not targeted for proteasomal degradation. Furthermore, invalidation of UBBP4 results in slower cell division, and accumulation of lamin A within the nucleolus. This implies that a subset of proteins reported as ubiquitin targets could rather be through these variants arising from wrongly annotated pseudogenes, and that there is a specificity in the modification and differences in the functional consequence of proteins modified by these new ubiquitin variants. The identification of additional ubiquitins thus entails a new layer of complexity in protein ubiquitylation that has been unnoticed until now.
HostingRepositoryPRIDE
AnnounceDate2020-03-19
AnnouncementXMLSubmission_2020-03-19_05:41:56.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnonym Anonym
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListcarbamoylated residue; ubiquitinylated lysine; 6x(13)C labeled residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02019-06-20 02:50:14ID requested
12020-03-19 05:41:57announced
Publication List
Dubois ML, Meller A, Samandi S, Brunelle M, Frion J, Brunet MA, Toupin A, Beaudoin MC, Jacques JF, L, é, vesque D, Scott MS, Lavigne P, Roucou X, Boisvert FM, UBB pseudogene 4 encodes functional ubiquitin variants. Nat Commun, 11(1):1306(2020) [pubmed]
Keyword List
submitter keyword: Ubiquitin, Pseudogene, Lamin A, Nucleolus
Contact List
Anonym Ous
contact affiliationAnonymous for double-blind review.
contact emailMikgree@Hotmail.com
lab head
Anonym Anonym
contact affiliationAnony
contact emailmikgree@hotmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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